Diabetes Unit, Department of Medicine, Surgery and Neurosciences, University of Siena, Siena, Italy; Fondazione Umberto Di Mario ONLUS c/o Toscana Life Science, Siena, Italy.
Diabetes Unit, Department of Medicine, Surgery and Neurosciences, University of Siena, Siena, Italy; Fondazione Umberto Di Mario ONLUS c/o Toscana Life Science, Siena, Italy; Tuscany Centre for Precision Medicine (CReMeP), Siena, Italy.
Cell Rep Med. 2024 Jun 18;5(6):101591. doi: 10.1016/j.xcrm.2024.101591. Epub 2024 Jun 4.
Circulating microRNAs (miRNAs) are linked to the onset and progression of type 1 diabetes mellitus (T1DM), thus representing potential disease biomarkers. In this study, we employed a multiplatform sequencing approach to analyze circulating miRNAs in an extended cohort of prospectively evaluated recent-onset T1DM individuals from the INNODIA consortium. Our findings reveal that a set of miRNAs located within T1DM susceptibility chromosomal locus 14q32 distinguishes two subgroups of individuals. To validate our results, we conducted additional analyses on a second cohort of T1DM individuals, confirming the identification of these subgroups, which we have named cluster A and cluster B. Remarkably, cluster B T1DM individuals, who exhibit increased expression of a set of 14q32 miRNAs, show better glycemic control and display a different blood immunomics profile. Our findings suggest that this set of circulating miRNAs can identify two different T1DM subgroups with distinct blood immunomics at baseline and clinical outcomes during follow-up.
循环 microRNAs（miRNAs）与 1 型糖尿病（T1DM）的发病和进展有关，因此代表了潜在的疾病生物标志物。在这项研究中，我们采用了多平台测序方法来分析来自 INNODIA 联盟的前瞻性评估的新诊断 T1DM 个体的扩展队列中的循环 miRNAs。我们的研究结果表明，一组位于 T1DM 易感染色体 14q32 位置的 miRNAs 将个体分为两个亚组。为了验证我们的结果，我们在第二个 T1DM 个体队列中进行了额外的分析，证实了这些亚组的鉴定，我们将其命名为簇 A 和簇 B。值得注意的是，簇 B 的 T1DM 个体表现出一组 14q32 miRNAs 的表达增加，其血糖控制更好，并表现出不同的血液免疫组学特征。我们的研究结果表明，这组循环 miRNAs 可以识别具有不同基线血液免疫组学和随访期间临床结局的两个不同的 T1DM 亚组。
