Department of Oral Biology, Lahore Medical and Dental College, Lahore, Pakistan.
Department of Prosthodontics, Lahore Medical and Dental College, Lahore, Pakistan.
J Coll Physicians Surg Pak. 2024 Jun;34(6):693-696. doi: 10.29271/jcpsp.2024.06.693.
To evaluate the effect of Ziziphus honey on the healing of post-extraction alveolar sockets by estimating the levels of osteopontin (OPN) in humans.
Randomised controlled trial. Place and Duration of the Study: Dental section of the Lahore General Hospital, Lahore, Pakistan, from March 2020 to February 2021.
A total of 30 patients were included in the study. The mean age was 35 ± 0.28 years. The participants were adults undergoing permanent molar extraction, randomly divided into two groups, a control group and an experimental group. After tooth extractions in both groups, 1ml of Ziziphus honey was administered into the extracted tooth socket of the experimental group while no intervention was done to the control group. Saliva samples were collected on day 0 before tooth extraction and on days 3 and 7 after tooth extractions. Enzyme-linked immunosorbent assay (ELISA) technique was used to measure the levels of OPN in the saliva sample. Radiographic evaluation was also done with the help of periapical radiographs using Image J® software. To find out the significance of the outcome in experimental and control groups, an unpaired t-test was applied. A p-value <0.05 was considered statistically significant.
A total of 30 participants were selected for the study, of which 16 were females and 14 were males. The OPN levels between the control vs. experimental groups were (22.55 ± 2.45 vs. 23.31 ± 2.38; p = 0.4) on day 0, (30.95 ± 2.96 vs. 53.29 ± 4.69; p = 0.001) on day 3, and (55.33 ± 4.52 vs. 81.90 ± 4.49; p = 0.001) on day 7.
Increased salivary levels of the OPN in the experimental group with the use of Ziziphus honey suggests better bone healing as compared to the control group.
Extraction tooth, Honey, Osteopontin, Ziziphus, Bone healing.
通过评估骨桥蛋白（OPN）水平，来评价酸枣蜜对拔牙后牙槽窝愈合的影响。
随机对照试验。研究地点和时间：巴基斯坦拉合尔总医院牙科，2020 年 3 月至 2021 年 2 月。
本研究纳入了 30 名患者。平均年龄为 35 ± 0.28 岁。参与者为接受恒磨牙拔牙的成年人，随机分为两组，对照组和实验组。两组拔牙后，实验组将 1ml 酸枣蜜注入拔牙窝，对照组不进行任何干预。在拔牙前 0 天、拔牙后 3 天和 7 天采集唾液样本。采用酶联免疫吸附试验（ELISA）技术测量唾液样本中的 OPN 水平。还使用 Image J®软件通过根尖片进行放射学评估。为了确定实验组和对照组结果的显著性，应用了非配对 t 检验。p 值<0.05 被认为具有统计学意义。
本研究共纳入 30 名患者，其中女性 16 名，男性 14 名。对照组和实验组的 OPN 水平分别为：第 0 天（22.55 ± 2.45 vs. 23.31 ± 2.38；p = 0.4），第 3 天（30.95 ± 2.96 vs. 53.29 ± 4.69；p = 0.001），第 7 天（55.33 ± 4.52 vs. 81.90 ± 4.49；p = 0.001）。
实验组使用酸枣蜜后，唾液中 OPN 水平升高，提示骨愈合情况优于对照组。
拔牙，蜂蜜，骨桥蛋白，酸枣，骨愈合。
