Turkmen Zeynep, Arslan Zeynep, Oka Merve, Yayla Murat, Bavunoglu Isil
Department of Science, Institute of Forensic Sciences and Legal Medicine, Istanbul University-Cerrahpaşa, Istanbul, Turkey.
Department of Internal Medicine, Cerrahpaşa Medical Faculty, Istanbul University-Cerrahpaşa, Istanbul, Turkey.
Drug Test Anal. 2025 Mar;17(3):412-419. doi: 10.1002/dta.3744. Epub 2024 Jun 5.
Olanzapine (OLZ), a second-generation antipsychotic drug, is effective in the treatment of acute psychosis, schizophrenia, agitation, bipolar mania, and other psychiatric problems. Antipsychotics are prescribed drugs, which lead the drug abuser to illegal methods of access. This behavior also demonstrates the association of OLZ with criminal involvement, commonly observed at forensic crime scenes. The acute toxicity and even death resulting from OLZ exposure have been highlighted in numerous studies. Therefore, developing analytical techniques to detect OLZ is essential for forensic toxicology. This study aimed to develop a specific and reliable LC-MS/MS method for OLZ detection and quantification in hair samples. The method was validated in terms of selectivity, linearity, limit of detection (LOD), limit of quantification (LOQ), trueness, precision, and uncertainty. The range of linearity was between 0.1-100 ng/mg, with LOD and LOQ values established at 0.036 ng/mg and 0.1 ng/mg, respectively. All validation results are within acceptable parameters. The validated method has been applied to authentic hair samples. The variation of OLZ concentrations in 12 hair segments (2 from Case 1 and 10 from Case 2) from two drug-positive patients, ranging from 0.131 to 0.460 ng/mg, is presented in this study. Although several studies have been conducted to determine OLZ in hair samples using segmental analysis via hair solubilization, this study is the first to determine OLZ in hair samples after "digestion" with comparative parameters prior to chromatographic analysis.
奥氮平（OLZ）是一种第二代抗精神病药物，对治疗急性精神病、精神分裂症、躁动、双相躁狂及其他精神问题有效。抗精神病药物属于处方药，这使得药物滥用者会通过非法途径获取。这种行为也表明OLZ与犯罪活动有关，这在法医犯罪现场很常见。众多研究都强调了因接触OLZ导致的急性毒性甚至死亡情况。因此，开发检测OLZ的分析技术对法医毒理学至关重要。本研究旨在开发一种用于检测和定量毛发样本中OLZ的特异性可靠的液相色谱 - 串联质谱（LC-MS/MS）方法。该方法在选择性、线性、检测限（LOD）、定量限（LOQ）、准确性、精密度和不确定度方面进行了验证。线性范围为0.1 - 100 ng/mg，LOD和LOQ值分别为0.036 ng/mg和0.1 ng/mg。所有验证结果均在可接受参数范围内。该经过验证的方法已应用于真实毛发样本。本研究展示了两名药物阳性患者的12个毛发段（病例1的2段和病例2的10段）中OLZ浓度的变化，范围为0.131至0.460 ng/mg。尽管已经进行了多项研究，通过毛发溶解的分段分析来测定毛发样本中的OLZ，但本研究是首次在色谱分析前通过具有比较参数的“消化”处理后测定毛发样本中的OLZ。
