Chen Xiulan, Lin Xin, Xu Lihuan, Liu Yu, Liu Xin, Zhang Chunhui, Xie Baosong
Department of Clinical Medicine, Fujian Medical University, Fuzhou, Fujian 350004, China.
Department of Respiratory and Critical Care Medicine, Fujian Provincial Geriatric Hospital, Fuzhou, Fujian 350009, China.
Open Life Sci. 2024 May 28;19(1):20220860. doi: 10.1515/biol-2022-0860. eCollection 2024.
The aim of this study is to observe the changes in autophagy activities in lung tissues of mice with different degrees of pulmonary fibrosis (PF), and explore the association between PF and autophagy activity. The PF model was established by bleomycin (BLM, 25 and 35 mg/kg) atomization inhalation in C57BL/6 mice, samples were collected on the 7, 14, and 28 days after BLM administration. Hematoxylin-eosin staining was used to observe the pathological changes in lung tissues. Masson staining was utilized to assess areas of blue collagen fiber deposition in lung tissues. Quantitative real time polymerase chain reaction was used to detect the mRNA expressions of autophagy-related genes, including Atg5, Atg7, and Atg10 in lung tissues. Western blot was used to detect the protein expressions of autophagy-related genes, including p62 and LC3II/LC3I in lung tissues. Compared with control group, BLM dose-dependently decreased PaO, mRNA expressions of Atg5, Atg7, Atg10, and LC3II/LC3I, while increased lung wet weight, lung coefficient, PF score, the blue area of collagen fibers, and p62 protein on the 7th, 14th, and 28th days. In conclusion, the more severe the PF induced by BLM, the lower the autophagy activity.
本研究旨在观察不同程度肺纤维化（PF）小鼠肺组织自噬活性的变化，并探讨PF与自噬活性之间的关联。通过对C57BL/6小鼠雾化吸入博来霉素（BLM，25和35mg/kg）建立PF模型，在给予BLM后的第7、14和28天采集样本。采用苏木精-伊红染色观察肺组织的病理变化。利用Masson染色评估肺组织中蓝色胶原纤维沉积区域。采用定量实时聚合酶链反应检测肺组织中自噬相关基因Atg5、Atg7和Atg10的mRNA表达。采用蛋白质免疫印迹法检测肺组织中自噬相关基因p62和LC3II/LC3I的蛋白表达。与对照组相比，BLM在第7、14和28天剂量依赖性降低PaO、Atg5、Atg7、Atg10和LC3II/LC3I的mRNA表达，同时增加肺湿重、肺系数、PF评分、胶原纤维蓝色面积和p62蛋白。综上所述，BLM诱导的PF越严重，自噬活性越低。
