Zhang Zhenyi, Li Yingchun, Wu Jian, Zhang Jihong, Chen Ning, Zhang Ning
Department of Rheumatology and Immunology, Shengjing Hospital of China Medical University, Shenyang, China.
College of Pharmacology, Harbin University of Commerce, Harbin, China.
Front Pharmacol. 2024 May 22;15:1415392. doi: 10.3389/fphar.2024.1415392. eCollection 2024.
Rheumatoid arthritis (RA) is a chronic autoimmune disease that affects the body. was a ethnic drug in China that was used to treat arthritis for hundreds of years. But, the therapeutic mechanism is so far unknown. Therefore, the chemical component and effect of on arthritis in rats were studied using HPLC-QTOF MS, micro-CT, and other experiments in this paper.
Male Sprague-Dawley rats were used to assess the activity. HPLC QTOF-MS was used to analyze the chemical profile of the (PF). Bovine type II collagen and Complete Freund's Adjuvant were used to stimulate and construct the collagen-induced arthritis (CIA) model. Three dosages of PF (100 mg/kg, 200 mg/kg, 400 mg/kg) were used to evaluate activity. Methotrexate was used as the positive drug. H/E staining and micro-CT methods were used to monitor the pathological changes of CIA rats. ELISA method was used to assess the serum level of immune- and inflammation-related cytokines. Immunohistochemical experiments were used to test the gene expression in JAK and Nf-κB pathways.
42 compounds were identified from PF. PF administration lowered the increased spleen index compared with that of control and MTX groups, and partially restored body weight, reduced paw swelling, and arthritis score compared with the model group. Macroscopic assessment indicated inflamed paw with significant swelling in the model group, while the extent of inflammation and swelling was attenuated by both MTX and PF. H/E staining experiments demonstrated that pathological changes of synovial cells and infiltration of inflammatory cells were observed in the model group. In contrast, the MTX and PF treatment partially reversed these pathological changes. Micro-CT examination showed severe injuries and scars caused by inflammation for the model group, and in the high-dosage group (400 mg/kg) the inflammation-caused injuries and scars were dramatically ameliorated. Mechanism study showed that PF restored Nf-κB phosphorylation and JAK2 expression compared with the model group.
possesses a potent effect on CIA rats. Nf-κB and JAK2 pathways are involved in its protective effect on CIA.
类风湿关节炎（RA）是一种影响身体的慢性自身免疫性疾病。[某种药物名称]是中国一种使用了数百年治疗关节炎的民族药物。但其治疗机制至今不明。因此，本文通过高效液相色谱-四极杆飞行时间质谱（HPLC-QTOF MS）、显微CT等实验研究了[某种药物名称]对大鼠关节炎的化学成分及作用。
使用雄性Sprague-Dawley大鼠评估[某种药物名称]的活性。采用HPLC QTOF-MS分析[某种药物名称]（PF）的化学特征。用牛II型胶原和完全弗氏佐剂刺激并构建胶原诱导性关节炎（CIA）模型。使用三种剂量的PF（100mg/kg、200mg/kg、400mg/kg）评估其活性。甲氨蝶呤用作阳性对照药物。采用苏木精-伊红（H/E）染色和显微CT方法监测CIA大鼠的病理变化。采用酶联免疫吸附测定（ELISA）法评估免疫和炎症相关细胞因子的血清水平。通过免疫组织化学实验检测JAK和Nf-κB信号通路中的基因表达。
从PF中鉴定出42种化合物。与对照组和甲氨蝶呤组相比，PF给药降低了升高的脾脏指数，与模型组相比部分恢复了体重、减轻了爪肿胀和关节炎评分。宏观评估表明模型组爪子发炎且明显肿胀，而甲氨蝶呤和PF均减轻了炎症和肿胀程度。H/E染色实验表明模型组观察到滑膜细胞的病理变化和炎性细胞浸润。相比之下，甲氨蝶呤和PF治疗部分逆转了这些病理变化。显微CT检查显示模型组有严重的炎症损伤和瘢痕，高剂量组（400mg/kg）炎症引起的损伤和瘢痕明显改善。机制研究表明，与模型组相比，PF恢复了Nf-κB磷酸化和JAK2表达。
[某种药物名称]对CIA大鼠有显著作用。Nf-κB和JAK2信号通路参与了其对CIA的保护作用。
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