使用Seralyzer Aris试剂条测试评估血清中茶碱的含量。

Determination of theophylline in serum with the Seralyzer Aris reagent strip test evaluated.

作者信息

Lindberg R, Ivaska K, Irjala K, Vanto T

出版信息

Clin Chem. 1985 Apr;31(4):613-4.

Abstract

We evaluated a new Seralyzer Aris reagent strip test (Ames Div., Miles Labs.) for the determination of theophylline in human serum. The method is based on the monoclonal enzyme immunoassay with dry reagent chemistry. The analysis is rapid and simple to perform: results are available only 5-10 min after receipt of the sample. Intra-assay precision (CV) was 2.2-3.3% (n = 15) for theophylline concentrations of 5-25 mg/L; interassay CV was 5.9% (n = 19) at 15 mg/L. The results (y) agreed well with those by liquid chromatography (x): r = 0.949 (p less than 0.001), and y = 0.967x + 0.214. We conclude the method is useful for rapid evaluation of theophylline concentrations in asthmatic patients.

摘要

我们评估了一种新的Seralyzer Aris试剂条检测法（米尔斯实验室艾姆斯分部）用于测定人血清中的茶碱。该方法基于采用干试剂化学的单克隆酶免疫测定法。分析快速且操作简单：收到样品后仅5 - 10分钟即可获得结果。对于5 - 25 mg/L的茶碱浓度，批内精密度（CV）为2.2 - 3.3%（n = 15）；在15 mg/L时，批间CV为5.9%（n = 19）。结果（y）与液相色谱法的结果（x）高度吻合：r = 0.949（p < 0.001），且y = 0.967x + 0.214。我们得出结论，该方法对于快速评估哮喘患者的茶碱浓度很有用。