Massoud N, Munzenberger P, Sarniak A
Am J Hosp Pharm. 1986 Jul;43(7):1722-6.
A reflectance photometry assay for measurement of serum theophylline concentration was evaluated by comparison with an enzyme-multiplied immunoassay technique (EMIT). The concentration of theophylline in blood samples obtained from patients receiving intravenous theophylline therapy in the pediatric intensive-care unit was measured by both the Seralyzer and the EMIT systems. The interday and intraday variability of each method were also determined by means of calibrators of known concentration (5 to 40 micrograms/mL). There was significant correlation between the serum theophylline concentrations determined by the Seralyzer system and the EMIT system. The overall standard error of the estimate was 2.3 micrograms/mL, but with operator experience this improved to 1.3 micrograms/mL. Intraday variability was 1.2 micrograms/mL for the Seralyzer and 0.7 micrograms/mL for EMIT; the respective values for interday variability were 1.4 micrograms/mL and 0.8 micrograms/mL. The Seralyzer method measures theophylline concentrations with reliability, convenience, and speed. It could be potentially useful in a satellite, clinic, or acute-care pharmacy area.
通过与酶放大免疫分析技术（EMIT）进行比较，对一种用于测定血清茶碱浓度的反射光度法进行了评估。使用Seralyzer系统和EMIT系统对从儿科重症监护病房接受静脉注射茶碱治疗的患者采集的血样中的茶碱浓度进行了测量。还通过已知浓度（5至40微克/毫升）的校准物确定了每种方法的日间和日内变异性。Seralyzer系统和EMIT系统测定的血清茶碱浓度之间存在显著相关性。估计的总体标准误差为2.3微克/毫升，但随着操作人员经验的增加，这一数值提高到了1.3微克/毫升。Seralyzer的日内变异性为1.2微克/毫升，EMIT为0.7微克/毫升；日间变异性的相应数值分别为1.4微克/毫升和0.8微克/毫升。Seralyzer方法能够可靠、便捷且快速地测定茶碱浓度。它在卫星诊所或急诊药房区域可能具有潜在用途。
