天冬氨酸氨甲酰基转移酶核苷酸复合物的3.0埃分辨率研究。
A 3.0-A resolution study of nucleotide complexes with aspartate carbamoyltransferase.
作者信息
Honzatko R B, Monaco H L, Lipscomb W N
出版信息
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5105-9. doi: 10.1073/pnas.76.10.5105.
Abstract
The binding sites of CTP, CDP, 5-BrCTP, and ATP to the allosteric site of aspartate carbamoyltransferase (carbamoylphosphate:L-aspartate carbamoyltransferase, EC 2.1.3.2) have been found in electron-density maps obtained at about 3 A resolution from x-ray diffraction studies of single crystals. The activator ATP binds in the anti conformation, whereas the inhibitor 5-BrCTP binds in the syn conformation. Both activator and inhibitor bind to the same local region of the enzyme. All of the cytidine nucleotides show important interactions of the base with the protein. The triphosphate conformations are similar, whereas the terminal phosphate of CDP occupies the site of the gamma-phosphate of CTP, thus implying a protein-nucleotide interaction at this site. These results are then related to biochemical studies.
摘要
通过对单晶进行X射线衍射研究，在分辨率约为3埃的电子密度图中发现了CTP、CDP、5-BrCTP和ATP与天冬氨酸氨甲酰基转移酶（氨甲酰磷酸：L-天冬氨酸氨甲酰基转移酶，EC 2.1.3.2）变构位点的结合位点。激活剂ATP以反式构象结合，而抑制剂5-BrCTP以顺式构象结合。激活剂和抑制剂都结合到酶的同一局部区域。所有胞苷核苷酸都显示出碱基与蛋白质的重要相互作用。三磷酸的构象相似，而CDP的末端磷酸占据CTP的γ-磷酸的位点，因此意味着在该位点存在蛋白质-核苷酸相互作用。然后将这些结果与生化研究联系起来。
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本文引用的文献
1
ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. III. THE INCORPORATION OF PYRIMIDINE AND PURINE ANALOGUES INTO DEOXYRIBONUCLEIC ACID.脱氧核糖核酸的酶促合成。III. 嘧啶和嘌呤类似物掺入脱氧核糖核酸的研究
Proc Natl Acad Sci U S A. 1958 Jul 15;44(7):633-40. doi: 10.1073/pnas.44.7.633.
2
The enzymology of control by feedback inhibition.反馈抑制控制的酶学
J Biol Chem. 1962 Mar;237:891-6.
3
4
Nucleoside conformations: an analysis of steric barriers to rotation about the glycosidic bond.核苷构象：围绕糖苷键旋转的空间位垒分析。
J Mol Biol. 1967 Jul 28;27(2):369-84. doi: 10.1016/0022-2836(67)90026-5.
5
Nuclear magnetic resonance study of metal-ion binding to adenosine triphosphate. II. Proton studies.金属离子与三磷酸腺苷结合的核磁共振研究。II. 质子研究。
J Chem Phys. 1965 Nov 1;43(9):3133-43. doi: 10.1063/1.1697287.
6
A proton magnetic resonance study of metal ion-adenine ring interactions in metal ion complexes with adenosine triphosphate.金属离子与三磷酸腺苷形成的金属离子配合物中金属离子 - 腺嘌呤环相互作用的质子磁共振研究。
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7
The purification of aspartate transcarbamylase of Escherichia coli and separation of its protein subunits.大肠杆菌天冬氨酸转氨甲酰酶的纯化及其蛋白质亚基的分离。
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10
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