大肠杆菌未结合天冬氨酸氨甲酰基转移酶在2.6埃分辨率下的结构
Structure of unligated aspartate carbamoyltransferase of Escherichia coli at 2.6-A resolution.
作者信息
Ke H M, Honzatko R B, Lipscomb W N
出版信息
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4037-40. doi: 10.1073/pnas.81.13.4037.
Abstract
The three-dimensional structure of the allosteric enzyme aspartate carbamoyltransferase (EC 2.1.3.2) has been refined to a crystallographic R-factor of 0.24 at 2.6-A resolution in the space group P321, where a and b are 122.1 A and c is 142.2 A. This structure is isomorphous to the form of the enzyme complexed to the allosteric inhibitor cytidine triphosphate. All sources of sequence information have been evaluated against the electron density. The corrected amino acid sequences of the catalytic and regulatory proteins have been incorporated in the model, and three regions in the active site are described: (i) near arginine-105, histidine-134, and arginine-167, (ii) near lysine-232 and arginine-229, and (iii) near lysine-83 and lysine-84.
摘要
变构酶天冬氨酸氨甲酰基转移酶（EC 2.1.3.2）的三维结构在空间群P321中已精修至2.6埃分辨率下晶体学R因子为0.24，其中a和b为122.1埃，c为142.2埃。该结构与和变构抑制剂三磷酸胞苷复合的酶形式同晶型。已根据电子密度评估了所有序列信息来源。催化蛋白和调节蛋白的校正氨基酸序列已纳入模型，并描述了活性位点的三个区域：（i）靠近精氨酸-105、组氨酸-134和精氨酸-167处，（ii）靠近赖氨酸-232和精氨酸-229处，以及（iii）靠近赖氨酸-83和赖氨酸-84处。
相似文献
1
Structure of unligated aspartate carbamoyltransferase of Escherichia coli at 2.6-A resolution.大肠杆菌未结合天冬氨酸氨甲酰基转移酶在2.6埃分辨率下的结构
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4037-40. doi: 10.1073/pnas.81.13.4037.
2
Structural consequences of effector binding to the T state of aspartate carbamoyltransferase: crystal structures of the unligated and ATP- and CTP-complexed enzymes at 2.6-A resolution.效应物与天冬氨酸氨甲酰基转移酶T态结合的结构后果：未结合配体以及结合ATP和CTP的酶在2.6埃分辨率下的晶体结构
Biochemistry. 1990 Aug 21;29(33):7691-701. doi: 10.1021/bi00485a019.
3
Crystal structure of the Glu-239----Gln mutant of aspartate carbamoyltransferase at 3.1-A resolution: an intermediate quaternary structure.天冬氨酸氨甲酰基转移酶的Glu-239----Gln突变体在3.1埃分辨率下的晶体结构：一种中间四级结构。
Proc Natl Acad Sci U S A. 1989 Nov;86(21):8212-6. doi: 10.1073/pnas.86.21.8212.
4
Three-dimensional structures of aspartate carbamoyltransferase from Escherichia coli and of its complex with cytidine triphosphate.来自大肠杆菌的天冬氨酸氨甲酰基转移酶及其与三磷酸胞苷复合物的三维结构。
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5276-80. doi: 10.1073/pnas.75.11.5276.
5
Complex of aspartate carbamoyltransferase from Escherichia coli with its allosteric inhibitor, cytidine triphosphate: electron density at 5.9-angstroms resolution.来自大肠杆菌的天冬氨酸氨甲酰基转移酶与其变构抑制剂三磷酸胞苷的复合物：5.9埃分辨率下的电子密度
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4437-41. doi: 10.1073/pnas.71.11.4437.
6
Molecular structure of Bacillus subtilis aspartate transcarbamoylase at 3.0 A resolution.枯草芽孢杆菌天冬氨酸转氨甲酰酶在3.0埃分辨率下的分子结构。
Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6087-91. doi: 10.1073/pnas.88.14.6087.
7
Arginine 54 in the active site of Escherichia coli aspartate transcarbamoylase is critical for catalysis: a site-specific mutagenesis, NMR, and X-ray crystallographic study.大肠杆菌天冬氨酸转氨甲酰酶活性位点中的精氨酸54对催化作用至关重要：一项定点诱变、核磁共振和X射线晶体学研究。
Protein Sci. 1992 Nov;1(11):1435-46. doi: 10.1002/pro.5560011105.
8
Structural asymmetry in the CTP-liganded form of aspartate carbamoyltransferase from Escherichia coli.来自大肠杆菌的天冬氨酸氨甲酰基转移酶CTP配体形式的结构不对称性。
J Mol Biol. 1987 Aug 20;196(4):853-75. doi: 10.1016/0022-2836(87)90410-4.
9
The pAR5 mutation and the allosteric mechanism of Escherichia coli aspartate carbamoyltransferase.大肠杆菌天冬氨酸氨甲酰基转移酶的pAR5突变与别构机制
EMBO J. 1987 Sep;6(9):2843-7. doi: 10.1002/j.1460-2075.1987.tb02581.x.
10
A 3.0-A resolution study of nucleotide complexes with aspartate carbamoyltransferase.天冬氨酸氨甲酰基转移酶核苷酸复合物的3.0埃分辨率研究。
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5105-9. doi: 10.1073/pnas.76.10.5105.
引用本文的文献
1
Novel Highlight in Malarial Drug Discovery: Aspartate Transcarbamoylase.疟疾药物发现的新亮点：天冬氨酸转氨甲酰酶。
Front Cell Infect Microbiol. 2022 Mar 4;12:841833. doi: 10.3389/fcimb.2022.841833. eCollection 2022.
2
Structure and mechanisms of Escherichia coli aspartate transcarbamoylase.大肠杆菌天冬氨酸转氨甲酰酶的结构与机制。
Acc Chem Res. 2012 Mar 20;45(3):444-53. doi: 10.1021/ar200166p. Epub 2011 Oct 19.
3
Crystallographic snapshots of the complete catalytic cycle of the unregulated aspartate transcarbamoylase from Bacillus subtilis.枯草芽孢杆菌未调节天冬氨酸转氨甲酰酶全催化循环的晶体学快照。
J Mol Biol. 2011 Aug 5;411(1):190-200. doi: 10.1016/j.jmb.2011.05.036. Epub 2011 May 31.
4
The first high pH structure of Escherichia coli aspartate transcarbamoylase.大肠杆菌天冬氨酸转氨甲酰酶的首个高pH值结构。
Proteins. 2009 Feb 1;74(2):318-27. doi: 10.1002/prot.22162.
5
Direct observation in solution of a preexisting structural equilibrium for a mutant of the allosteric aspartate transcarbamoylase.对别构天冬氨酸转氨甲酰酶突变体预先存在的结构平衡在溶液中的直接观察。
Proc Natl Acad Sci U S A. 2007 Jan 9;104(2):495-500. doi: 10.1073/pnas.0607641104. Epub 2007 Jan 3.
6
Transcriptional response of Escherichia coli to TPEN.大肠杆菌对TPEN的转录反应。
J Bacteriol. 2006 Sep;188(18):6709-13. doi: 10.1128/JB.00680-06.
7
Gene expression regulation by the Curli activator CsgD protein: modulation of cellulose biosynthesis and control of negative determinants for microbial adhesion.卷曲纤维激活蛋白CsgD对基因表达的调控：纤维素生物合成的调节及微生物黏附负调控因子的控制
J Bacteriol. 2006 Mar;188(6):2027-37. doi: 10.1128/JB.188.6.2027-2037.2006.
8
Replacement of Asp-162 by Ala prevents the cooperative transition by the substrates while enhancing the effect of the allosteric activator ATP on E. coli aspartate transcarbamoylase.将天冬氨酸-162替换为丙氨酸可阻止底物的协同转变，同时增强变构激活剂ATP对大肠杆菌天冬氨酸转氨甲酰酶的作用。
Protein Sci. 2002 May;11(5):1074-81. doi: 10.1110/ps.4500102.
9
Allosteric regulation of catalytic activity: Escherichia coli aspartate transcarbamoylase versus yeast chorismate mutase.催化活性的变构调节：大肠杆菌天冬氨酸转氨甲酰酶与酵母分支酸变位酶的比较
Microbiol Mol Biol Rev. 2001 Sep;65(3):404-21, table of contents. doi: 10.1128/MMBR.65.3.404-421.2001.
10
Binding of bisubstrate analog promotes large structural changes in the unregulated catalytic trimer of aspartate transcarbamoylase: implications for allosteric regulation.双底物类似物的结合促进了天冬氨酸转氨甲酰酶非调节性催化三聚体的巨大结构变化：对别构调节的影响。
Proc Natl Acad Sci U S A. 2000 May 9;97(10):5077-82. doi: 10.1073/pnas.090087197.
本文引用的文献
1
ASPARTATE TRANSCARBAMYLASE, AN ENZYME DESIGNED FOR FEEDBACK INHIBITION.天冬氨酸转氨甲酰酶，一种为反馈抑制而设计的酶。
Fed Proc. 1964 May-Jun;23:727-35.
2
The enzymology of control by feedback inhibition.反馈抑制控制的酶学
J Biol Chem. 1962 Mar;237:891-6.
3
Gross quaternary changes in aspartate carbamoyltransferase are induced by the binding of N-(phosphonacetyl)-L-aspartate: A 3.5-A resolution study.N-（膦酰乙酰基）-L-天冬氨酸的结合诱导了天冬氨酸氨甲酰基转移酶的四级结构显著变化：一项3.5埃分辨率的研究。
Proc Natl Acad Sci U S A. 1982 May;79(10):3125-8. doi: 10.1073/pnas.79.10.3125.
4
5
Crystal and molecular structures of native and CTP-liganded aspartate carbamoyltransferase from Escherichia coli.来自大肠杆菌的天然及CTP配体结合的天冬氨酸氨甲酰基转移酶的晶体结构和分子结构
J Mol Biol. 1982 Sep 15;160(2):219-63. doi: 10.1016/0022-2836(82)90175-9.
6
Location of amino acid alterations in mutants of aspartate transcarbamoylase: Structural aspects of interallelic complementation.天冬氨酸转氨甲酰酶突变体中氨基酸改变的位置：等位基因间互补的结构方面
Proc Natl Acad Sci U S A. 1984 Jan;81(1):115-9. doi: 10.1073/pnas.81.1.115.
7
Amino acid sequence of the catalytic subunit of aspartate transcarbamoylase from Escherichia coli.来自大肠杆菌的天冬氨酸转氨甲酰酶催化亚基的氨基酸序列。
Proc Natl Acad Sci U S A. 1983 May;80(9):2467-71. doi: 10.1073/pnas.80.9.2467.
8
Nucleotide sequence of the structural gene (pyrB) that encodes the catalytic polypeptide of aspartate transcarbamoylase of Escherichia coli.编码大肠杆菌天冬氨酸转氨甲酰酶催化多肽的结构基因（pyrB）的核苷酸序列。
Proc Natl Acad Sci U S A. 1983 May;80(9):2462-6. doi: 10.1073/pnas.80.9.2462.
9
Interactions of phosphate ligands with Escherichia coli aspartate carbamoyltransferase in the crystalline state.磷酸配体与结晶态大肠杆菌天冬氨酸氨甲酰基转移酶的相互作用。
J Mol Biol. 1982 Sep 15;160(2):265-86. doi: 10.1016/0022-2836(82)90176-0.
10
Crystallographic determination of symmetry of aspartate transcarbamylase.天冬氨酸转氨甲酰酶对称性的晶体学测定
Nature. 1968 Jun 22;218(5147):1119-21. doi: 10.1038/2181119a0.
Zotero 插件