Guo Aijiao, Song Menglin, Chen Qichen, Zhang Ziwei, Feng Ye, Hu Xialin, Liu Meichuan
School of Chemical Science and Engineering, Shanghai Key Lab of Chemical Assessment and Sustainability, Tongji University, 1239 Siping Road, Shanghai 200092, China.
Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Anal Chem. 2024 Jun 18;96(24):9944-9952. doi: 10.1021/acs.analchem.4c01157. Epub 2024 Jun 6.
Label-free photoelectrochemical sensors have the advantages of high sensitivity and a simple electrode structure. However, its performance is greatly limited due to the photoactive materials' weak photoactivity and poor stability. Herein, a robust homogeneous photoelectrochemical (PEC) aptasensor has been constructed for atrazine (ATZ) based on photoetching (PE) surface oxygen vacancies (O)-enriched Bismuth vanadate (BiVO) (PE-BVO). The surface of the O improves the carrier separation ability of BiVO, thus providing a superior signal substrate for the sensor. A thiol molecular layer self-assembled on PE-BVO acts as a blocker, while 2D graphene acts as a signal-on probe after release from the aptamer-graphene complex. The fabricated sensor has a wide linear detection range of 0.5 pM to 10.0 nM and a low detection limit of 0.34 pM (S/N = 3) for ATZ. In addition, it can efficiently work in a wide pH range (3-13) and high ionic strength (∼6 M Na), which provides promising opportunities for detecting environmental pollutants under complex conditions.
无标记光电化学传感器具有灵敏度高和电极结构简单的优点。然而，由于光活性材料的光活性较弱和稳定性较差，其性能受到很大限制。在此，基于光蚀刻（PE）富表面氧空位（O）的钒酸铋（BiVO）（PE-BVO）构建了一种用于阿特拉津（ATZ）的稳健的均相光电化学（PEC）适配体传感器。O的表面提高了BiVO的载流子分离能力，从而为传感器提供了优异的信号底物。自组装在PE-BVO上的硫醇分子层起阻断作用，而二维石墨烯从适配体-石墨烯复合物释放后作为信号开启探针。所制备的传感器对ATZ具有0.5 pM至10.0 nM的宽线性检测范围和0.34 pM（S/N = 3）的低检测限。此外，它可以在宽pH范围（3-13）和高离子强度（~6 M Na）下有效工作，这为在复杂条件下检测环境污染物提供了广阔的前景。
