Zimmermann B, Barrach H J, Merker H J, Hinz N
Eur J Cell Biol. 1985 Jan;36(1):66-73.
In high density cultures of mouse fetal lung cells, so-called "mass cultures", development of organoid structures, formation of a basement membrane (BM), and differentiation of pneumocytes type II occur accompanied by synthesis and secretion of lamellar bodies. The relationship between the formation of a BM, on the one hand, and morphogenesis as well as differentiation of pneumocytes type II, on the other hand, has been investigated by use of antibodies against BM components in the lung mass culture. It is shown here that anti-laminin antibodies prevented BM formation, but morphogenesis and pneumocyte differentiation occurred as in untreated cultures. Short-term treatment with the antibody revealed that the BM is formed only during the first 2 to 3 days in vitro. Already formed BM could not be removed by anti-laminin. Anti-collagen type IV antibodies showed no effect in the lung mass culture except for a stronger staining of the BM. Anti-BM-1 antibodies caused no changes in morphogenesis, cell differentiation and BM formation either, but the mesenchymal intercellular space exhibited a dark staining, which is probably due to antigen-antibody complexes. The results obtained with anti-laminin antibodies indicate that a BM is not necessary for lung cell differentiation in vitro.
在小鼠胎儿肺细胞的高密度培养中，即所谓的“大规模培养”，类器官结构的发育、基底膜（BM）的形成以及II型肺细胞的分化伴随着板层小体的合成与分泌而发生。通过在肺大规模培养中使用针对BM成分的抗体，研究了一方面BM的形成与另一方面II型肺细胞的形态发生和分化之间的关系。此处显示，抗层粘连蛋白抗体可阻止BM形成，但形态发生和肺细胞分化与未处理的培养物一样发生。用该抗体进行短期处理表明，BM仅在体外的头2至3天内形成。已形成的BM不能被抗层粘连蛋白去除。抗IV型胶原抗体在肺大规模培养中未显示出任何作用，只是对BM的染色更强。抗BM-1抗体在形态发生、细胞分化和BM形成方面也未引起任何变化，但间充质细胞间空间呈现深色染色，这可能是由于抗原-抗体复合物所致。用抗层粘连蛋白抗体获得的结果表明，体外肺细胞分化不需要BM。
