School of Dentistry, Sao Paulo University, Av. Do Café-Subsetor Oeste-11, Ribeirão Preto, SP, 14040-904, Brazil.
School of Dentistry, Federal University of Goiás, Av. Universitária, s/n-Setor Leste Universitário, Goiânia, GO, 74605-020, Brazil.
Sci Rep. 2024 Jun 6;14(1):13066. doi: 10.1038/s41598-024-58559-w.
The aim of this study was to assess the surface and tissue quality of keratinized mucosa grafts (KMG) obtained using the conventional scalpel and mucotome techniques. This was an experimental in vitro/ex vivo study involving six porcine hemi-mandibles. Specimens were harvested using both the mucotome and conventional scalpel techniques, with randomization determining the choice of technique for tissue removal. The specimens were prepared following predefined laboratory protocols and subsequently subjected to optical microscopy for evaluating epithelial and connective tissue and scanning electron microscopy for topographical and 3D profilometry analysis. Tissues harvested using the mucotome exhibited a linear base and uniform thickness, along with the presence of submucosa and fibrous connective tissue, all of which are ideal for graft success. Differences in the surface characteristics of specimens obtained through the two techniques were observed during a comparative analysis of images obtained through both microscopy types. KMG obtained using the mucotome technique displayed greater uniformity and reduced undesirable cell presence compared to the scalpel technique, thereby enhancing the likelihood of success in soft tissue graft surgical procedures. This study provides valuable insights to oral healthcare professionals and may contribute to future research aimed at achieving more successful surgeries, shorter postoperative recovery times, reduced discomfort, and an overall more positive patient experience.
本研究旨在评估使用传统手术刀和黏膜切开刀技术获取的角化黏膜移植物（KMG）的表面和组织质量。这是一项涉及六个猪半下颌骨的体外/体内实验研究。标本分别使用黏膜切开刀和传统手术刀技术采集，随机决定组织去除的技术选择。标本按照预设的实验室方案进行准备，然后进行光学显微镜检查以评估上皮和结缔组织，以及扫描电子显微镜检查以进行形貌和 3D 轮廓分析。使用黏膜切开刀采集的组织具有线性基底和均匀的厚度，同时存在黏膜下层和纤维结缔组织，所有这些都是移植物成功的理想条件。在对两种显微镜类型获得的图像进行比较分析时，观察到两种技术获得的标本表面特征存在差异。与手术刀技术相比，使用黏膜切开刀技术获得的 KMG 具有更高的均匀性和更少的不良细胞存在，从而提高了软组织移植手术成功的可能性。本研究为口腔保健专业人员提供了有价值的见解，并可能为未来旨在实现更成功手术、更短的术后恢复时间、减少不适和总体更积极的患者体验的研究做出贡献。
