School of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, China.
Tianjin Key Laboratory of Animal Molecular Breeding and Biotechnology, Tianjin Engineering Research Center of Animal Healthy Farming, Institute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin, China.
Anim Sci J. 2024 Jan-Dec;95(1):e13966. doi: 10.1111/asj.13966.
Prolonged exposure of bisphenol A (BPA) has adverse effects on in vitro maturation (IVM) of oocytes, but treatment with tauroursodeoxycholic acid (TUDCA) can improve the IVM and development of embryos. The purpose of this study was to investigate the effects of BPA and both BPA and TUDCA on IVM and parthenogenetic development of embryos. The results showed that BPA treatment adverse effects on the cumulus expansion index, survival rate, polar body rate, mitochondrial distribution of the oocytes after maturation culture, and that it also decreased the cleavage rate and blastocyst rate of embryos after parthenogenetic develpoment. In addition, BPA treatment upregulated expression of genes related to endoplasmic reticulum stress and apoptosis and increased the intracellular reactive oxygen species (ROS) level, while it decreased expression of genes related to cumulus expansion. However, the supplementation of TUDCA relieved these adverse effects of BPA except polar body rate, blastocyst rate, and expression of BCL2 and PTGS1. In conclusion, the supplementation of TUDCA can partly attenuate the negative effects of BPA on IVM and parthenogenetic development of embryos, possibly by modification of the expression of genes related to endoplasmic reticulum stress, apoptosis and cumulus expansion, intracellular ROS level, and mitochondrial distribution.
双酚 A（BPA）的长期暴露对卵母细胞的体外成熟（IVM）有不良影响，但牛磺熊脱氧胆酸（TUDCA）的治疗可以改善 IVM 和胚胎的发育。本研究的目的是探讨 BPA 以及 BPA 和 TUDCA 对卵母细胞 IVM 和孤雌胚胎发育的影响。结果表明，BPA 处理对卵母细胞成熟培养后的卵丘扩展指数、存活率、极体率、线粒体分布有不良影响，还降低了孤雌胚胎发育后的卵裂率和囊胚率。此外，BPA 处理上调了与内质网应激和细胞凋亡相关的基因表达，增加了细胞内活性氧（ROS）水平，同时降低了与卵丘扩展相关的基因表达。然而，TUDCA 的补充缓解了 BPA 对 IVM 和孤雌胚胎发育的除极体率、囊胚率、BCL2 和 PTGS1 表达以外的其他不良影响。总之，TUDCA 的补充可以部分减轻 BPA 对卵母细胞 IVM 和孤雌胚胎发育的负面影响，可能是通过调节与内质网应激、细胞凋亡和卵丘扩展、细胞内 ROS 水平和线粒体分布相关的基因表达。
