Kalligosfyri Panagiota M, Cinti Stefano
Department of Pharmacy, University of Naples "Federico II", 80131 Naples, Italy.
BAT Center - Interuniversity Center for Studies on Bioinspired Agro- Environmental Technology, University of Naples "Federico II", 80055 Naples, Italy.
Anal Chem. 2024 Jun 18;96(24):9773-9779. doi: 10.1021/acs.analchem.4c02032. Epub 2024 Jun 6.
In analytical chemistry, preconcentration represents a critical step able to enhance the accuracy of detection; however, the experimental procedures needed to preconcentrate samples might be characterized by drawbacks regarding the whole analytical process, e.g., being complex, invasive, and/or time-consuming. In this study, a novel 3D paper-based origami device is introduced for multifold analyte preconcentration. Leveraging the benefits of paper-based substrates, the proposed architecture boosts sample preconcentration while minimizing time and tasks for measurements, solely by exploiting the porous and versatile nature of paper-based substrates. In comparison with other paper-based approaches reported in the literature for preconcentration, the present architecture offers the ability to be programmed for obtaining the needed sensitivity increase without sacrificing measurement time. To demonstrate the efficacy of the novel approach, the 3D paper-based origami device was deeply characterized, including the most relevant parameters, i.e., disk size and number, unfolding time, and volume, and subsequently applied for the preconcentration and the detection of various analytes in real matrices, namely, mercury in tap water and glucose in sweat, resulting in a 400% and 300% sensitivity enhancement, respectively. This innovative preconcentration tool addresses the limitations of existing conventional methods, providing increased sensitivity without the use of expensive and time-consuming procedures through only exploiting the intrinsic properties of paper-based substrates and a rationale design. The proposed architecture emerges as a universal tool to be adopted and programmed for various analytical systems and fields of application.
在分析化学中，预富集是提高检测准确性的关键步骤；然而，样品预富集所需的实验程序可能存在一些缺点，例如整个分析过程复杂、侵入性强和/或耗时。在本研究中，引入了一种新型的基于纸张的3D折纸装置用于多次分析物预富集。利用基于纸张的基质的优点，所提出的架构提高了样品预富集效率，同时仅通过利用基于纸张的基质的多孔性和通用性，将测量时间和任务减至最少。与文献中报道的其他用于预富集的基于纸张的方法相比，当前架构能够进行编程，以在不牺牲测量时间的情况下获得所需的灵敏度提高。为了证明这种新方法的有效性，对基于纸张的3D折纸装置进行了深入表征，包括最相关的参数，即圆盘尺寸和数量、展开时间和体积，随后将其应用于实际基质中各种分析物的预富集和检测，即自来水中的汞和汗液中的葡萄糖，灵敏度分别提高了400%和300%。这种创新的预富集工具克服了现有传统方法的局限性，仅通过利用基于纸张的基质的固有特性和合理设计，在不使用昂贵且耗时的程序的情况下提高了灵敏度。所提出的架构成为一种通用工具，可用于各种分析系统和应用领域并进行编程。
