Gipson I K, Friend J, Spurr S J
Invest Ophthalmol Vis Sci. 1985 Apr;26(4):425-33.
Sheets of corneal epithelium removed from 9-mm buttons of adult rabbit corneas using Dispase II were placed on abraded (basement membrane intact) or keratectomized corneas of anesthetized rabbits. Both types of wounds extended from limbus to limbus. The host animals were maintained under deep anesthesia for 3 hr, during which time culture medium was dripped onto the surface of the transplant. A soft contact lens then was placed over the cornea and the eye bandaged shut. Short-term experiments indicated that after 24 hr the transplanted epithelium was adherent to both abraded and keratectomized corneas (n = 4). Hemidesmosomes had formed between basal cells of donor epithelium and denuded host basement membrane, and cytoplasmic blebs had extended from donor epithelium into host keratectomized stroma. Seven transplants to abraded corneas and 17 transplants to keratectomized corneas were followed for longer time periods. Six of the seven transplants to abraded corneas were maintained until termination of the experiment (four at 4 weeks, one at 2 weeks, one at 1 week). Three of the 17 transplants to keratectomized corneas were maintained until termination (one at 4 weeks, one at 2 weeks, and one at 6 days). The remaining 14 sloughed between days 2 and 6. These data indicate that it is feasible to transplant corneal epithelial sheets and that they can be maintained most successfully if the host basement membrane is present.
使用Dispase II从成年兔角膜的9毫米纽扣状组织上取下的角膜上皮片，被放置在麻醉兔的擦伤（基底膜完整）或角膜切除的角膜上。两种类型的伤口均从角膜缘延伸至角膜缘。宿主动物在深度麻醉下维持3小时，在此期间将培养基滴加到移植表面。然后在角膜上放置一个软性隐形眼镜，并将眼睛包扎起来。短期实验表明，24小时后移植的上皮细胞附着在擦伤和角膜切除的角膜上（n = 4）。供体上皮细胞的基底细胞与裸露的宿主基底膜之间形成了半桥粒，并且细胞质泡从供体上皮细胞延伸到宿主角膜切除的基质中。对7个移植到擦伤角膜的组织和17个移植到角膜切除角膜的组织进行了更长时间的观察。7个移植到擦伤角膜的组织中有6个维持到实验结束（4个在4周，1个在2周，1个在1周）。17个移植到角膜切除角膜的组织中有3个维持到实验结束（1个在4周，1个在2周，1个在6天）。其余14个在第2天至第6天之间脱落。这些数据表明移植角膜上皮片是可行的，并且如果宿主基底膜存在，它们可以最成功地维持。
