具有抗Li + 蜜二糖载体的大肠杆菌突变体。
Escherichia coli mutants possessing an Li+-resistant melibiose carrier.
作者信息
Shiota S, Yamane Y, Futai M, Tsuchiya T
出版信息
J Bacteriol. 1985 Apr;162(1):106-9. doi: 10.1128/jb.162.1.106-109.1985.
Abstract
Escherichia coli K-12 strains in the absence of the lactose carrier grew on the disaccharide melibiose as the sole source of carbon. The presence of 0.1 mM Li+ in the medium strongly inhibited growth of such cells, and Li+-resistant mutants appeared after several days of incubation. These mutants showed altered cation coupling to melibiose transport via the melibiose carrier. Cotransport between H+ and melibiose was lost in the mutants, although Na+-melibiose cotransport was retained. We observed no Li+-melibiose cotransport. Therefore, these mutants represent a new type of cation-coupling mutants of the melibiose carrier.
摘要
在缺乏乳糖载体的情况下，大肠杆菌K - 12菌株能够以二糖蜜二糖作为唯一碳源生长。培养基中存在0.1 mM的Li +会强烈抑制此类细胞的生长，孵育几天后出现了Li +抗性突变体。这些突变体显示出通过蜜二糖载体与蜜二糖转运的阳离子偶联发生了改变。尽管保留了Na + - 蜜二糖共转运，但在突变体中H +与蜜二糖之间的共转运丧失了。我们未观察到Li + - 蜜二糖共转运。因此，这些突变体代表了蜜二糖载体的一种新型阳离子偶联突变体。
相似文献
1
Escherichia coli mutants possessing an Li+-resistant melibiose carrier.具有抗Li + 蜜二糖载体的大肠杆菌突变体。
J Bacteriol. 1985 Apr;162(1):106-9. doi: 10.1128/jb.162.1.106-109.1985.
2
Altered cation coupling to melibiose transport in mutants of Escherichia coli.大肠杆菌突变体中阳离子与蜜二糖转运偶联的改变。
J Biol Chem. 1982 Aug 10;257(15):8902-6.
3
Na+-coupled transport of melibiose in Escherichia coli: analysis of mutants with altered cation specificity.大肠杆菌中蜜二糖的钠偶联转运：阳离子特异性改变的突变体分析。
Tokai J Exp Clin Med. 1982;7 Suppl:61-4.
4
Melibiose transport of Escherichia coli.大肠杆菌的蜜二糖转运
J Bacteriol. 1980 Mar;141(3):1031-6. doi: 10.1128/jb.141.3.1031-1036.1980.
5
H + -substrate cotransport by the melibiose membrane carrier in Escherichia coli.大肠杆菌中蜜二糖膜载体介导的H⁺-底物共转运
Membr Biochem. 1980;3(1-2):131-46. doi: 10.3109/09687688009063881.
6
Effect of lithium ion on melibiose transport in Escherichia coli.锂离子对大肠杆菌中蜜二糖转运的影响。
J Membr Biol. 1978 Jul 21;42(1):45-59. doi: 10.1007/BF01870393.
7
Transport properties of Asp-51-->Glu and Asp-120-->Glu mutants of the melibiose carrier of Escherichia coli.大肠杆菌蜜二糖载体的天冬氨酸-51→谷氨酸和天冬氨酸-120→谷氨酸突变体的转运特性
Biochim Biophys Acta. 1994 Mar 23;1190(2):225-30. doi: 10.1016/0005-2736(94)90078-7.
8
Membrane transport of p-nitrophenyl-alpha-galactoside by the melibiose carrier of Escherichia coli.对硝基苯基-α-半乳糖苷通过大肠杆菌蜜二糖载体的膜转运
J Membr Biol. 1980 Sep 30;56(2):169-75. doi: 10.1007/BF01875968.
9
Cation coupling to melibiose transport in Salmonella typhimurium.鼠伤寒沙门氏菌中阳离子与蜜二糖转运的偶联
J Bacteriol. 1980 Oct;144(1):192-9. doi: 10.1128/jb.144.1.192-199.1980.
10
Melibiose-cation cotransport system of Escherichia coli.大肠杆菌的蜜二糖-阳离子共转运系统
Ann N Y Acad Sci. 1985;456:342-9. doi: 10.1111/j.1749-6632.1985.tb14884.x.
引用本文的文献
1
Arg-52 in the melibiose carrier of Escherichia coli is important for cation-coupled sugar transport and participates in an intrahelical salt bridge.大肠杆菌蜜二糖载体中的精氨酸-52对阳离子偶联的糖转运很重要，并参与螺旋内盐桥的形成。
J Bacteriol. 1999 Oct;181(20):6377-86. doi: 10.1128/JB.181.20.6377-6386.1999.
2
Proline transport in Salmonella typhimurium: putP permease mutants with altered substrate specificity.鼠伤寒沙门氏菌中的脯氨酸转运：底物特异性改变的putP通透酶突变体
J Bacteriol. 1986 Nov;168(2):590-4. doi: 10.1128/jb.168.2.590-594.1986.
3
Dissecting the molecular mechanism of ion-solute cotransport: substrate specificity mutations in the putP gene affect the kinetics of proline transport.剖析离子-溶质协同转运的分子机制：putP基因中的底物特异性突变影响脯氨酸转运的动力学。
J Membr Biol. 1991 May;121(3):201-14. doi: 10.1007/BF01951554.
4
Asp-51 and Asp-120 are important for the transport function of the Escherichia coli melibiose carrier.天冬氨酸-51和天冬氨酸-120对于大肠杆菌蜜二糖载体的转运功能很重要。
J Bacteriol. 1992 May;174(9):3083-6. doi: 10.1128/jb.174.9.3083-3086.1992.
5
Cloning and sequencing of the melB gene encoding the melibiose permease of Salmonella typhimurium LT2.鼠伤寒沙门氏菌LT2蜜二糖通透酶编码基因melB的克隆与测序
Mol Gen Genet. 1992 Jul;234(1):74-80. doi: 10.1007/BF00272347.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Altered cation coupling to melibiose transport in mutants of Escherichia coli.大肠杆菌突变体中阳离子与蜜二糖转运偶联的改变。
J Biol Chem. 1982 Aug 10;257(15):8902-6.
3
Melibiose transport of Escherichia coli.大肠杆菌的蜜二糖转运
J Bacteriol. 1980 Mar;141(3):1031-6. doi: 10.1128/jb.141.3.1031-1036.1980.
4
Inhibitory effect of Li+ on cell growth and pyruvate kinase activity of Escherichia coli.锂对大肠杆菌细胞生长和丙酮酸激酶活性的抑制作用。
J Bacteriol. 1984 Nov;160(2):812-4. doi: 10.1128/jb.160.2.812-814.1984.
5
Escherichia coli mutants with altered cation recognition by the melibiose carrier.通过蜜二糖载体改变阳离子识别的大肠杆菌突变体。
J Bacteriol. 1984 Oct;160(1):445-7. doi: 10.1128/jb.160.1.445-447.1984.
6
Lithium ion-sugar cotransport via the melibiose transport system in Escherichia coli. Measurement of Li+ transport and specificity.锂离子通过大肠杆菌中的蜜二糖转运系统与糖共转运。锂离子转运及特异性的测定。
J Biol Chem. 1983 Nov 10;258(21):12765-7.
7
8
A sodium-dependent sugar co-transport system in bacteria.细菌中的一种钠依赖性糖共转运系统。
Biochem Biophys Res Commun. 1971 Jul 2;44(1):132-8. doi: 10.1016/s0006-291x(71)80168-7.
9
Galactoside transport dissociated from proton movement in mutants of Escherichia coli.在大肠杆菌突变体中，半乳糖苷转运与质子运动相分离。
Biochem Biophys Res Commun. 1973 Jan 23;50(2):551-8. doi: 10.1016/0006-291x(73)90875-9.
10
A mutant of Escherichia coli K 12 energy-uncoupled for lactose transport.大肠杆菌K12的一种乳糖转运能量解偶联突变体。
Biochim Biophys Acta. 1972 Mar 17;255(3):786-97. doi: 10.1016/0005-2736(72)90391-4.
Zotero 插件