Shimizu Kaori, Saito Taizo, Takashima Yasuhiro, Okada Haruhiko, Asakawa Mitsuhiko, Inoshima Yasuo
Joint Department of Veterinary Medicine, Gifu University, Gifu, Gifu 501-1193, Japan.
Education and Research Center for Food Animal Health, Gifu University (GeFAH), Gifu, Gifu 501-1193, Japan.
J Nematol. 2024 May 25;56(1):20240022. doi: 10.2478/jofnem-2024-0022. eCollection 2024 Mar.
Mermithidae is a family of nematodes that parasitize a wide range of invertebrates worldwide. Herein, we report nematodes that were unexpectedly found in three of 486 adult stable flies () captured from three farms (F1, F2, and F3) in different regions of Gifu Prefecture, Japan. We aimed to characterize these nematodes both at the morphological and molecular level. Morphological studies revealed that the nematodes were juveniles of Mermithidae. Phylogenetic analysis based on 18S and 28S rDNA indicated that the mermithids from farms F1 and F2 could be categorized into the same cluster as and sp., whereas the mermithid from farm F3 clustered with sp. Additionally, these mermithids could be categorized within the same clusters as related mermithids detected in Japan that parasitize various arthropod orders. Our findings suggest that these stable flies may have been parasitized by mermithids already present in the region and that genetically distinct species of mermithids occur across Japan. To the best of our knowledge, this is the first report of mermithids parasitizing adult stable flies in Japan.
索线虫科是一类寄生于全球多种无脊椎动物的线虫。在此，我们报告在从日本岐阜县不同地区的三个农场（F1、F2和F3）捕获的486只成年厩螫蝇中有三只意外发现了线虫。我们旨在从形态学和分子水平对这些线虫进行特征描述。形态学研究表明这些线虫是索线虫科的幼虫。基于18S和28S核糖体DNA的系统发育分析表明，来自农场F1和F2的索线虫可与 和 种归入同一类群，而来自农场F3的索线虫与 种聚类在一起。此外，这些索线虫可与在日本检测到的寄生于各种节肢动物目的相关索线虫归入同一类群。我们的研究结果表明，这些厩螫蝇可能已被该地区已有的索线虫寄生，并且在日本各地存在遗传上不同的索线虫物种。据我们所知，这是日本关于索线虫寄生成年厩螫蝇的首次报告。
