Yokomori Rui, Kusakabe Takehiro G, Nakai Kenta
Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.
Institute for Integrative Neurobiology, Graduate School of Natural Science, Konan University, Kobe 658-8501, Japan.
NAR Genom Bioinform. 2024 Jun 6;6(2):lqae067. doi: 10.1093/nargab/lqae067. eCollection 2024 Jun.
-splicing is a post-transcriptional processing event that joins exons from separate RNAs to produce a chimeric RNA. However, the detailed mechanism of -splicing remains poorly understood. Here, we characterize -spliced genes and provide insights into the mechanism of -splicing in the tunicate . Tunicates are the closest invertebrates to humans, and their genes frequently undergo -splicing. Our analysis revealed that, in genes that give rise to both -spliced and non--spliced messenger RNAs, -splice acceptor sites were preferentially located at the first functional acceptor site, and their paired donor sites were weak in both and humans. Additionally, we found that -spliced genes had GU- and AU-rich 5' transcribed regions. Our data and findings not only are useful for research community, but may also aid in a better understanding of the -splicing mechanism, potentially advancing the development of gene therapy based on -splicing.
-剪接是一种转录后加工事件，它将来自不同RNA的外显子连接起来以产生嵌合RNA。然而，-剪接的详细机制仍知之甚少。在这里，我们对-剪接基因进行了表征，并深入了解了被囊动物中-剪接的机制。被囊动物是与人类亲缘关系最近的无脊椎动物，它们的基因经常发生-剪接。我们的分析表明，在产生-剪接和非-剪接信使RNA的基因中，-剪接受体位点优先位于第一个功能性接受体位点，并且它们配对的供体位点在被囊动物和人类中都较弱。此外，我们发现-剪接基因具有富含GU和AU的5'转录区域。我们的数据和发现不仅对-研究领域有用，还可能有助于更好地理解-剪接机制，潜在地推动基于-剪接的基因治疗的发展。
