Liu Qianwen, Zhou Lingling, Xin Siyu, Yang Qingli, Wu Wei, Hou Xiudan
College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.
Food Chem X. 2024 May 16;22:101471. doi: 10.1016/j.fochx.2024.101471. eCollection 2024 Jun 30.
A poly (ionic liquid) enhanced poly(acrylamide-acrylic acid) (PIL-PAM/AA) hydrogel-based colorimetric sensor was designed to detect zearalenone (ZEN). Different AuPt nanoparticles were synthesized via the on-pot method. Through the kinetic analysis and the theoretical calculation, AuPt possessed the relatively low energy barriers to adsorb and decompose HO so that it exhibited relatively better catalytic activity (K = 2.02 × 10, V = 6.14 × 10). AuPt nanoparticles were encapsulated into PIL-PAM/AA hydrogel via the interaction between aptamer and cDNA. In the presence of ZEN, the embedded AuPt nanoparticles were released to complete the catalytic reaction. Coupled with the smartphone application, the established method provided the linear range of 1-250 ng mL, with a detection limit of 0.6979 ng mL for ZEN. Meanwhile, it also possessed excellent selectivity and good anti-interference performance. In wheat and corn samples, spiked recoveries were ranging from 75% to 113.30%.
设计了一种基于聚（离子液体）增强聚（丙烯酰胺 - 丙烯酸）（PIL - PAM/AA）水凝胶的比色传感器来检测玉米赤霉烯酮（ZEN）。通过一锅法合成了不同的金铂纳米颗粒。通过动力学分析和理论计算，金铂对吸附和分解羟基具有相对较低的能垒，因此表现出相对较好的催化活性（K = 2.02×10，V = 6.14×10）。通过适体与互补DNA之间的相互作用将金铂纳米颗粒封装到PIL - PAM/AA水凝胶中。在存在玉米赤霉烯酮的情况下，嵌入的金铂纳米颗粒被释放以完成催化反应。结合智能手机应用程序，所建立的方法提供了1 - 250 ng/mL的线性范围，玉米赤霉烯酮的检测限为0.6979 ng/mL。同时，它还具有出色的选择性和良好的抗干扰性能。在小麦和玉米样品中，加标回收率在75%至113.30%之间。
