Department of Neurosurgery, The Fourth People's Hospital of Jinan, Jinan, Shandong, China.
Department of Neurology, The Third Affiliated Hospital of Shandong First Medical University, Jinan, China.
Toxicol Mech Methods. 2024 Oct;34(8):908-919. doi: 10.1080/15376516.2024.2364191. Epub 2024 Jun 10.
In this work, we have analyzed the neuroprotective activity of marrubiin against MPTP-induced Parkinson's disease (PD) in rat brains. MPTP (1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine) a neurotoxin was administered intraperitoneally (i.p.,) to rats and then treated using marrubiin. After marrubiin treatment, rats were trained, and tested for behavioral analyses like cognitive performance, open field test, rotarod test, grip strength test, beam walking test, the status of body weight, and striatal levels of neurotransmitters like dopamine, norepinephrine, serotonin, DOPAC, homovanillic acid, 5-hydroxy indole acetic acid, the status of oxidative stress markers like LPO, protein carbonyl content (PCC), Xanthine oxidase (XO), and status of antioxidant enzyme levels like SOD, CAT, GPX in the striatum and hippocampal tissues, status of neuroinflammatory markers like TNF-α, IL1β, IL-6, and status of histological architecture in brain striatum were also analyzed. All these parameters were significantly ( < 0.05) abnormal in MPTP-induced rats. Marrubiin (MB) treated shows significant ( < 0.05) near normal behavioral restoration in cognitive performance, open field, rotarod, grip strength, and beam walking tests. Furthermore, the status of body weight, and levels of neurotransmitters, were also significantly ( < 0.05) reversed to near normalcy in marrubiin-treated rats. Similarly, oxidative stress, antioxidant enzyme levels in the striatum and hippocampal tissues, TNF-α, IL1β, IL-6 levels, and histological architecture were noted to be restored to near normalcy in marrubiin-treated rats. Collectively, our preliminary results highlight the neuroprotective ability of marrubiin. However, the cellular and biochemical mechanisms of marrubiin's neuroprotective ability have to be studied in detail.
在这项工作中，我们分析了 marrubiin 对 MPTP 诱导的大鼠脑帕金森病 (PD) 的神经保护活性。MPTP（1-甲基-4-苯基-1,2,3,6-四氢吡啶）是一种神经毒素，通过腹腔内（i.p.）给予大鼠，然后用 marrubiin 进行治疗。在 marrubiin 治疗后，对大鼠进行训练，并进行行为分析，如认知表现、旷场试验、转棒试验、握力试验、走棒试验、体重状况以及纹状体中的神经递质，如多巴胺、去甲肾上腺素、血清素、DOPAC、高香草酸、5-羟色胺乙酸、氧化应激标志物，如 LPO、蛋白羰基含量 (PCC)、黄嘌呤氧化酶 (XO) 和纹状体和海马组织中的抗氧化酶水平，如 SOD、CAT、GPX、神经炎症标志物，如 TNF-α、IL1β、IL-6 和大脑纹状体的组织学结构状况也进行了分析。所有这些参数在 MPTP 诱导的大鼠中均显著（<0.05）异常。Marrubiin（MB）治疗后，在认知表现、旷场、转棒、握力和走棒试验中，行为恢复明显（<0.05）接近正常。此外，体重状况和神经递质水平在 marrubiin 治疗的大鼠中也明显（<0.05）恢复到接近正常。同样，氧化应激、纹状体和海马组织中的抗氧化酶水平、TNF-α、IL1β、IL-6 水平以及组织学结构在 marrubiin 治疗的大鼠中也恢复到接近正常。总之，我们的初步结果强调了 marrubiin 的神经保护能力。然而，marrubiin 的神经保护能力的细胞和生化机制仍需详细研究。
