College of Nursing, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China.
College of Nursing, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China.
Neuroscience. 2024 Jul 23;551:299-306. doi: 10.1016/j.neuroscience.2024.05.027. Epub 2024 Jun 6.
This study aimed to investigate whether moxibustion could affect PI3K/Akt pathway to regulate Transforming acidic coiled-coil containing protein 3 (TACC3) and promote axonal regeneration to improve learning and memory function in middle cerebral artery occlusion (MCAO) rats.
Sixty SD rats were randomly divided into 4 groups: sham-operated control group (SC), model control group (MC), model + moxibustion group (MM), and model + inhibitor + moxibustion group (MIM). The rats in MC, MM, and MIM groups were made into MCAO models, and PI3K inhibitor LY294002 was injected into the rats in MIM group before modeling; while the rats in SC group were only treated with artery separation without monofilament inserting. After that, the rats in MM and MIM groups were intervented with moxibustion. We used the Zea-Longa scale, micro-Magnetic Resonance Imaging (micro-MRI), Morris water maze (MWM), TUNEL, western blot (WB), immunofluorescence and immunohistochemistry to evaluate the neurological deficits, cerebral infarct volume, learning and memory, apoptotic cell percentage in the hippocampal, the expression level of axonal regeneration and PI3K/AKt related proteins, the expression level of TACC3. The detection of 2 h after surgery showed the result before moxibustion and 7 days after the intervention showed the results after moxibustion.
After 7 d of intervention, the scores of Zea-Longa and the cerebral infarct volume, the escape latency, the percentage of apoptosis cells of MM group were lower than that of MC and MIM groups; the frequency of rats crossed the previous platform location, PI3K, p-Akt/t-Akt and TACC3, the level of GAP-43 in MM group was more than MC and MIM groups (P < 0.05). While no statistical difference existed between MIM group and MC group (P > 0.05).
Moxibustion can promote axonal regeneration and improve learning and memory of Post-stroke cognitive impairment via activating the PI3K/AKT signaling pathway and TACC3.
本研究旨在探讨艾灸是否可以通过调节磷酸肌醇 3-激酶/蛋白激酶 B（PI3K/Akt）通路来影响转化酸性卷曲螺旋蛋白 3（TACC3），从而促进轴突再生，改善大脑中动脉闭塞（MCAO）大鼠的学习记忆功能。
将 60 只 SD 大鼠随机分为 4 组：假手术对照组（SC）、模型对照组（MC）、模型+艾灸组（MM）和模型+抑制剂+艾灸组（MIM）。MC、MM 和 MIM 组大鼠制作 MCAO 模型，MIM 组大鼠在建模前注射 PI3K 抑制剂 LY294002；SC 组大鼠仅进行动脉分离，不插入单丝。然后，MM 和 MIM 组大鼠进行艾灸干预。采用 Zea-Longa 评分、微磁共振成像（micro-MRI）、Morris 水迷宫（MWM）、TUNEL、western blot（WB）、免疫荧光和免疫组化评估神经功能缺损、脑梗死体积、学习记忆、海马区凋亡细胞比例、轴突再生和 PI3K/Akt 相关蛋白表达水平、TACC3 表达水平。术后 2h 检测显示艾灸前结果，干预 7d 后显示艾灸后结果。
干预 7d 后，MM 组的 Zea-Longa 评分及脑梗死体积、逃避潜伏期、凋亡细胞比例均低于 MC 和 MIM 组；MM 组穿越原平台位置的大鼠频率、PI3K、p-Akt/t-Akt 和 TACC3、GAP-43 水平均高于 MC 和 MIM 组（P<0.05）。而 MIM 组与 MC 组比较差异无统计学意义（P>0.05）。
艾灸可通过激活 PI3K/Akt 信号通路和 TACC3，促进轴突再生，改善缺血性脑卒中后认知障碍大鼠的学习记忆功能。
