Hunan Cancer Hospital & The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, China.
3D Medicines Inc., Shanghai 201114, China.
Genomics. 2024 Jul;116(4):110875. doi: 10.1016/j.ygeno.2024.110875. Epub 2024 Jun 5.
Exploration of a stably expressed gene as a reference is critical for the accurate evaluation of miRNAs isolated from small extracellular vesicles (sEVs). In this study, we analyzed small RNA sequencing on plasma sEV miRNAs in the training dataset (n = 104) and found that miR-140-3p was the most stably expressed candidate reference for sEV miRNAs. We further demonstrated that miR-140-3p expressed most stably in the validation cohort (n = 46) when compared to two other reference miRNAs, miR-451a and miR-1228-3p, and the commonly-used miRNA reference U6. Finally, we compared the capability of miR-140-3p and U6 as the internal reference for sEV miRNA expression by evaluating key miRNAs expression in lung cancer patients and found that miR-140-3p was more suitable as a sEV miRNA reference gene. Taken together, our data indicated miR-140-3p as a stable internal reference miRNA of plasma sEVs to evaluate miRNA expression profiles in lung cancer patients.
探索稳定表达的基因对于准确评估从小细胞外囊泡 (sEV) 中分离出的 miRNAs 至关重要。在本研究中，我们分析了训练数据集（n=104）中血浆 sEV miRNAs 的小 RNA 测序，发现 miR-140-3p 是 sEV miRNAs 最稳定表达的候选参考物。我们进一步证明，与另外两个参考 miRNA（miR-451a 和 miR-1228-3p）和常用的 miRNA 参考物 U6 相比，miR-140-3p 在验证队列（n=46）中表达最稳定。最后，我们通过评估肺癌患者中关键 miRNAs 的表达，比较了 miR-140-3p 和 U6 作为 sEV miRNA 表达内参的能力，发现 miR-140-3p 更适合作为 sEV miRNA 参考基因。综上所述，我们的数据表明 miR-140-3p 是一种稳定的血浆 sEV 内参 miRNA，可用于评估肺癌患者的 miRNA 表达谱。
