对患有坏死性小肠结肠炎或自发性肠穿孔的婴儿肠组织进行胃肠道病原体的分子检测。
Molecular testing for gastrointestinal pathogens in intestinal tissue of infants with necrotizing enterocolitis or spontaneous intestinal perforation.
机构信息
Department of Pediatrics, Avera Research Institute and University of South Dakota Sanford School of Medicine, Sioux Falls, SD, USA.
Department of Pediatrics, Division of Neonatology, Nationwide Children's Hospital and The Ohio State University College of Medicine, Columbus, OH, USA.
出版信息
J Perinatol. 2024 Dec;44(12):1755-1761. doi: 10.1038/s41372-024-01999-z. Epub 2024 Jun 7.
OBJECTIVE
The objective of this study was to determine the frequency of common gastrointestinal bacterial, parasitic, and viral pathogen detection in necrotizing enterocolitis (NEC) or spontaneous intestinal perforation (SIP) -associated intestinal tissue.
STUDY DESIGN
Retrospective cohort study examined formalin fixed, paraffin embedded (FFPE) surgical or autopsy intestinal tissue from NEC or SIP specimens. DNA and RNA were extracted and analyzed by multiplex PCR panel (GIFA Biofire). DNA or RNA from stool samples containing each pathogen were extracted for positive controls.
RESULTS
The total number of intestinal tissue samples were 193 from 310 infants (156 NEC, 37 SIP). Six (3%) infants with stage III NEC tested positive for a target pathogen; 2, C. difficile; 3, Enteroaggregtive E. coli; and 1, Giardia. No gastrointestinal viral pathogens were detected.
CONCLUSION
Molecular testing yielded few GI pathogens suggesting that these organisms are likely not major causes or facilitators of NEC or SIP.
目的
本研究旨在确定坏死性小肠结肠炎（NEC）或自发性肠穿孔（SIP）相关肠组织中常见胃肠道细菌、寄生虫和病毒病原体的检测频率。
研究设计
回顾性队列研究检查了来自 NEC 或 SIP 标本的福尔马林固定、石蜡包埋（FFPE）手术或尸检肠组织。通过多重 PCR 面板（GIFA Biofire）提取和分析 DNA 和 RNA。从含有每种病原体的粪便样本中提取 DNA 或 RNA 作为阳性对照。
结果
共有 310 名婴儿的 193 个肠组织样本；156 例 NEC，37 例 SIP。6 名（3%）III 期 NEC 婴儿的目标病原体检测呈阳性；2 例艰难梭菌；3 例肠聚集性大肠杆菌；1 例贾第虫。未检测到胃肠道病毒病原体。
结论
分子检测结果显示很少有胃肠道病原体，这表明这些病原体不太可能是 NEC 或 SIP 的主要病因或促进因素。
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