Strosznajder J, Radominska-Pyrek A, Horrocks L A
Biochim Biophys Acta. 1979 Jul 27;574(1):48-56. doi: 10.1016/0005-2760(79)90083-3.
Substantial activities of cholinephosphotransferase (EC 2.7.8.2) and ethanolaminephosphotransferase (EC 2.7.8.1) were found with lysed synaptosomes but not with intact synaptosomes isolated from adult rat brains. Synaptosomal and non-synaptosomal microsomal transferases were similar in kinetic properties. Substantial activities of synaptosomal transferases have not been described previously. Part of the glycerophospholipids in synaptosomal membranes may be synthesized in the nerve ending in addition to the glycerophospholipids supplied by axonal transport. The synthesis of the alkylacyl type of choline and ethanolamine glycerophospholipids was moderately inhibited by 1 mM ATP and 1 microM cyclic AMP. This synthesis was also inhibited by more than 50% by 1 mM norepinephrine and to a lesser extent by 5 mM hydroxytryptamine and 1 mM acetylcholine. Cyclic AMP may mediate the effects of biogenic amines. The relative synthesis of different glycerophospholipid classes and the relative proportion of alkylacyl type (plasmalogen precursors) and diacyl type of glycerophospholipids may be influenced by the levels of adenine nucleotides and/or biogenic amines. Elevated cyclic AMP levels will decrease the synthesis of plasmalogen precursors.
在裂解的突触体中发现了大量的胆碱磷酸转移酶（EC 2.7.8.2）和乙醇胺磷酸转移酶（EC 2.7.8.1）的活性，但从成年大鼠脑中分离出的完整突触体中未发现。突触体和非突触体微粒体转移酶在动力学性质上相似。突触体转移酶的大量活性以前未曾描述过。除了轴突运输提供的甘油磷脂外，突触体膜中的部分甘油磷脂可能在神经末梢合成。1 mM ATP和1 microM环磷酸腺苷适度抑制胆碱和乙醇胺甘油磷脂的烷基酰基类型的合成。1 mM去甲肾上腺素也可抑制该合成超过50%，5 mM 5-羟色胺和1 mM乙酰胆碱的抑制作用较小。环磷酸腺苷可能介导生物胺的作用。腺嘌呤核苷酸和/或生物胺的水平可能影响不同甘油磷脂类别的相对合成以及甘油磷脂的烷基酰基类型（缩醛磷脂前体）和二酰基类型的相对比例。环磷酸腺苷水平升高会降低缩醛磷脂前体的合成。
