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基于非靶向 GC-MS 代谢组学的不同品种籼稻种子差异。

Non-targeted GC-MS metabolomics-based differences in Indica rice seeds of different varieties.

机构信息

Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, China.

School of Nursing, Jiangxi University of Chinese Medicine, Nanchang, 330004, China.

出版信息

BMC Plant Biol. 2024 Jun 8;24(1):519. doi: 10.1186/s12870-024-05255-6.

DOI:10.1186/s12870-024-05255-6
PMID:38851682
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11162567/
Abstract

Rice seeds of different varieties exhibited distinct metabolic profiles in our study. We analyzed the metabolites in seeds of six rice varieties (CH, HM, NX, YX, HY, and MX) using non-targeted GC-MS. Our findings revealed that amino acids, sugars, and organic acids were predominant in all varieties, with significant differences observed in CH compared to the others. Specifically phenylalanine and glycine content differed notably in NX and YX, respectively. Additionally, 1,5-anhydroglucitol content in NX, and glutamate, aspartate, and lactulose in NX, YX, HM, HY, and MX were up-regulated. Due to the biological functions of these amino acids and sugars, these indicated that compared to CH, rice of NX were more conducive to metabolism of carbohydrate and fat, and healthy growth maintenance in the human body, but mightThese variations suggest that NX rice may be more beneficial for carbohydrate and fat metabolism and overall health maintenance compared to CH. However, it may not be suitable for diabetic patients. YX rice may not be an ideal glycine supplement, rice ofwhile HM, HY, and MX rice could serve as potential lactulose sources. Furthermore, NX and YX rice exhibited higher levels of main storage proteins compared to CH. This study offers valuable insights into the metabolic differences among various rice varieties.

摘要

在本研究中,不同品种的水稻种子表现出明显不同的代谢特征。我们使用非靶向 GC-MS 分析了六种水稻品种(CH、HM、NX、YX、HY 和 MX)种子中的代谢物。我们的研究结果表明,在所有品种中,氨基酸、糖和有机酸是主要的代谢物,与其他品种相比,CH 中有显著差异。具体来说,NX 和 YX 中的苯丙氨酸和甘氨酸含量差异显著。此外,NX 中的 1,5-脱水葡萄糖醇含量以及 NX、YX、HM、HY 和 MX 中的谷氨酸、天冬氨酸和乳果糖含量上调。由于这些氨基酸和糖的生物学功能,这表明与 CH 相比,NX 水稻更有利于碳水化合物和脂肪的代谢以及人体的健康生长维持,但可能不适合糖尿病患者。YX 水稻可能不是甘氨酸的理想补充剂,而 HM、HY 和 MX 水稻则可能是潜在的乳果糖来源。此外,NX 和 YX 水稻的主要储存蛋白含量高于 CH。本研究为不同水稻品种的代谢差异提供了有价值的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/627f/11162567/afe080e3426b/12870_2024_5255_Fig7_HTML.jpg
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