Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan, 430079, PR China.
Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan, 430079, PR China.
Biosens Bioelectron. 2024 Oct 1;261:116470. doi: 10.1016/j.bios.2024.116470. Epub 2024 Jun 5.
The aggravation of antibiotic resistance genes (ARGs) in the environment has posed a significant global health crisis. Accurate evaluation of ARGs levels in a facile manner is a pressing issue for environmental surveillance. Here, we demonstrate a unique dumbbell-shaped cascade nanozyme for visual/photoelectrochemical (PEC) dual-mode detection of ARGs. Gold nanoparticles (AuNPs) with tunable exposed facets are controllably anchored onto ZIF-8 dodecahedrons, exhibiting glucose oxidase (GOx)-like (ZIF-8@Au/G) and peroxidase (POD)-like (ZIF-8@Au/P) activities. Upon the occurrence of ARGs, an asymmetric cascade-amplified "dumbbell" configuration is spontaneously generated via target-induced DNA hybridization, comprising GOx-like ZIF-8@Au/G with capture DNA on one side and POD-like ZIF-8@Au/P with signal DNA on the opposite side. Such a cascade nano-system can efficiently oxidize colorless 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) into its green oxidation state and synergistically decompose HO, realizing colorimetric/PEC dual-mode ARGs detection with a detection limit of 0.112 nM. The applicability of the present bioassay is validated through measuring ARGs in real sludge samples. This work suggests the possibility to rationally design task-specific nanozymes and develop target-responsive nano-cascade assays for environmental monitoring.
抗生素耐药基因 (ARGs) 在环境中的加剧对全球健康构成了重大威胁。以简单的方式准确评估环境中 ARGs 的水平是环境监测的当务之急。在这里，我们展示了一种独特的哑铃形级联纳米酶，用于抗生素耐药基因的可视化/光电化学 (PEC) 双模式检测。具有可调暴露面的金纳米颗粒 (AuNPs) 被可控地锚定在 ZIF-8 十二面体上，表现出葡萄糖氧化酶 (GOx)-样 (ZIF-8@Au/G) 和过氧化物酶 (POD)-样 (ZIF-8@Au/P) 活性。当出现 ARGs 时，通过目标诱导的 DNA 杂交自发产生不对称级联放大的“哑铃”结构，包括一侧带有捕获 DNA 的 GOx 样 ZIF-8@Au/G 和另一侧带有信号 DNA 的 POD 样 ZIF-8@Au/P。这种级联纳米系统可以有效地将无色 2,2'-联氮双 (3-乙基苯并噻唑啉-6-磺酸) 氧化为其绿色氧化态，并协同分解 HO，实现比色/PEC 双模式 ARGs 检测，检测限为 0.112 nM。通过测量实际污泥样品中的 ARGs，验证了本生物测定法的适用性。这项工作表明了合理设计特定任务的纳米酶和开发针对目标的纳米级联分析的可能性，用于环境监测。
