School of Environmental Engineering, Henan University of Technology, Lianhua Road 100#, Zhengzhou 450001, Henan Province, People's Republic of China.
School of Environmental Engineering, Henan University of Technology, Lianhua Road 100#, Zhengzhou 450001, Henan Province, People's Republic of China; JIANGSU YUYUE KAILITE BIOTECHNOLOGY Co., LTD., Danyang, Baisheng Road1#, Zhenjiang 212300, Jiangsu Province, People's Republic of China.
Bioelectrochemistry. 2024 Dec;160:108750. doi: 10.1016/j.bioelechem.2024.108750. Epub 2024 May 28.
Overuse of enrofloxacin (ENR) has posed a potential threat to ecosystems and public health, so it is critical to sensitive and accurate determination of ENR residues. In this work, a novel ultra-sensitive and specific electrochemical aptasensor was fabricated based on the cobalt diselenide loaded gold and platinum nanoflowers (Au@Pt NFs/ CoSe) and Exonuclease III (Exo III)-assisted cycle amplification strategy for the detection of ENR. Au@Pt NFs/ CoSe nanosheets as the substrate material, with large surface area, accelerate electron transfer and attach more DNA probes on the electrode substrate, have effectively enhanced the electrochemical performance of the electrode. With the existence of Enrofloxacin (ENR), the aptamer recognizes and binds to ENR, thus the signal probe cDNA was released and immobilized onto the electrode surface to hybridized with methylene blue (MB) labelled DNA (MB-DNA), thereby triggering the Exo III-assisted cycle for further signal amplification. As expected, the prepared aptasensor demonstrated excellent sensitivity and selectivity, with a wide linear range from 5.0 × 10 ng/mL to 1.0 × 10 ng/mL for ENR, a low detection limit of 1.59 × 10 ng/mL. Consequently, this strategy provided a promising avenue for ultrasensitive and accurate detection of ENR in milk samples.
过度使用恩诺沙星（ENR）对生态系统和公众健康构成了潜在威胁，因此，对 ENR 残留进行敏感和准确的测定至关重要。在这项工作中，基于负载二硒化钴的金和铂纳米花（Au@Pt NFs/CoSe）和核酸外切酶 III（Exo III）辅助循环扩增策略，制备了一种新型超灵敏和特异的电化学适体传感器，用于检测 ENR。Au@Pt NFs/CoSe 纳米片作为基底材料，具有较大的表面积，加速了电子转移并在电极基底上附着了更多的 DNA 探针，从而有效提高了电极的电化学性能。存在恩诺沙星（ENR）时，适体识别并与 ENR 结合，从而使信号探针 cDNA 被释放并固定在电极表面上与亚甲基蓝（MB）标记的 DNA（MB-DNA）杂交，从而引发 Exo III 辅助循环进行进一步的信号放大。不出所料，所制备的适体传感器表现出优异的灵敏度和选择性，对 ENR 的线性范围从 5.0×10 ng/mL 到 1.0×10 ng/mL，检测限低至 1.59×10 ng/mL。因此，该策略为牛奶样品中 ENR 的超灵敏和准确检测提供了一种有前途的方法。
