Graduated Program in Genetics, Conservation, and Evolutionary Biology (PPG GCBEv), National Institute for Amazon Research (INPA), Manaus, AM, Brazil.
Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil.
Toxicon. 2024 Aug 2;246:107797. doi: 10.1016/j.toxicon.2024.107797. Epub 2024 Jun 7.
The Brazilian Amazon is home to a rich fauna of scorpion species of medical importance, some of them still poorly characterized regarding their biological actions and range of clinical symptoms after envenoming. The Amazonian scorpion species Tityus strandi and Tityus dinizi constitute some of the scorpions in this group, with few studies in the literature regarding their systemic repercussions. In the present study, we characterized the clinical, inflammatory, and histopathological manifestations of T. strandi and T. dinizi envenoming in a murine model using Balb/c mice. The results show a robust clinical response based on clinical score, hyperglycemia, leukocytosis, increased cytokines, and histopathological changes in the kidneys and lungs. Tityus strandi envenomed mice presented more prominent clinical manifestations when compared to Tityus dinizi, pointing to the relevance of this species in the medical scenario, with both species inducing hyperglycemia, leukocytosis, increased cytokine production in the peritoneal lavage, increased inflammatory infiltrate in the lungs, and acute tubular necrosis after T. strandi envenoming. The results presented in this research can help to understand the systemic manifestations of scorpion accidents in humans caused by the target species of the study and point out therapeutic strategies in cases of scorpionism in remote regions of the Amazon.
巴西亚马逊地区拥有丰富的医学重要性蝎子物种，其中一些在其生物作用和中毒后的临床症状范围方面仍未得到充分描述。亚马逊蝎子物种 Tityus strandi 和 Tityus dinizi 是该类蝎子中的一些，关于它们的全身反应的文献研究很少。在本研究中，我们使用 Balb/c 小鼠在小鼠模型中对 T. strandi 和 T. dinizi 的中毒进行了临床、炎症和组织病理学表现的特征描述。结果表明，根据临床评分、高血糖、白细胞增多、细胞因子增加和肾脏和肺部的组织病理学变化，表现出强烈的临床反应。与 Tityus dinizi 相比，Tityus strandi 中毒的小鼠表现出更明显的临床症状，这表明该物种在医学场景中的相关性，两种物种都会导致高血糖、白细胞增多、腹腔灌洗液中细胞因子产生增加、肺部炎症浸润增加以及 T. strandi 中毒后的急性肾小管坏死。本研究中的结果可以帮助理解由研究目标物种引起的人类蝎子事故的全身表现，并指出在亚马逊偏远地区发生蝎子中毒时的治疗策略。
