Soma M, Manku M S, Jenkins D K, Horrobin D F
Prostaglandins. 1985 Feb;29(2):323-33. doi: 10.1016/0090-6980(85)90212-6.
To clarify the metabolism of PGE2, prostacyclin (PGI2) and thromboxane A2 (TxA2) in small vessels in spontaneously hypertensive rats (SHR), we removed superior mesenteric vascular beds from 10 week old SHR and age matched normotensive controls (WKY). The mesenteric artery was perfused with Krebs-Henseleit buffer and samples of effluent collected every 15 minutes during 3 hours perfusion for analysis of PGE2, 6-keto-PGF1 alpha (a stable metabolite of PGI2) and TxB2 (a stable metabolite of TxA2) by specific radioimmunoassays. Levels of all three arachidonic acid (AA) metabolites, PGE2, 6-keto-PGF1 alpha and TxB2, in the mesenteric effluent were significantly reduced in SHR as compared to WKY. TxB2 was detected in all samples throughout the perfusion. 6-keto-PGF1 alpha/PGE2 ratios and TxB2/PGE2 ratios were significantly increased in SHR. 6-keto-PGF1 alpha/TxB2 ratios in the first four samples were significantly decreased in SHR as compared to WKY. These data suggest that there may be reduced availability of PG precusor AA and unbalanced synthesis of PGs in small vessels in SHR. Both may have relevance to the development of hypertension in the animals.
为阐明自发性高血压大鼠（SHR）小血管中前列腺素E2（PGE2）、前列环素（PGI2）和血栓素A2（TxA2）的代谢情况，我们从10周龄的SHR和年龄匹配的正常血压对照大鼠（WKY）身上取下肠系膜上血管床。用Krebs-Henseleit缓冲液灌注肠系膜动脉，并在3小时灌注过程中每隔15分钟收集流出液样本，通过特异性放射免疫分析法分析PGE2、6-酮-前列腺素F1α（PGI2的稳定代谢产物）和血栓素B2（TxA2的稳定代谢产物）。与WKY相比，SHR肠系膜流出液中所有三种花生四烯酸（AA）代谢产物PGE2、6-酮-前列腺素F1α和血栓素B2的水平均显著降低。在整个灌注过程中，所有样本均检测到血栓素B2。SHR中6-酮-前列腺素F1α/PGE2比值和血栓素B2/PGE2比值显著升高。与WKY相比，SHR前四个样本中的6-酮-前列腺素F1α/血栓素B2比值显著降低。这些数据表明，SHR小血管中PG前体AA的可用性可能降低，PG合成失衡。两者可能都与动物高血压的发生有关。
