State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Yangling 712100, Shaanxi, China.
College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China.
Plant Dis. 2024 Oct;108(10):3072-3077. doi: 10.1094/PDIS-03-24-0546-RE. Epub 2024 Sep 18.
In recent years, kiwifruit viral diseases have become increasingly prevalent in kiwifruit-producing regions of China, significantly impacting both the yield and quality of kiwifruit. This has emerged as a significant constraint on the healthy and sustainable development of the kiwifruit industry. The use of virus-free propagation materials has been proven the most effective strategy for controlling plant viral diseases. In the present study, shoot tip culture, shoot tip cryotherapy, and their combinations with thermotherapy were established to eradicate Actinidia virus A (AcVA), Actinidia virus B (AcVB), and Actinidia chlorotic ringspot-associated virus (AcCRaV) from . Additionally, the impact of shoot tip size on virus eradication was evaluated. Among the three confirmed viruses, regardless of the procedure, AcVB was the easiest to eradicate, followed by AcVA and AcCRaV. Combining thermotherapy with shoot tip culture or cryotherapy resulted in a higher virus-free frequency (up to 27.3 and 50%, respectively) than shoot tip culture or cryotherapy alone (0 to 20%). Notably, the combination of thermotherapy and 0.5- to 1-mm shoot tip cryotherapy was shown to be the most effective protocol for virus eradication from . , which produced 50% of regenerated shoots free from all the tested viruses. To the best of our knowledge, this is the first report on virus elimination from kiwifruit infected with multiple viruses based on conventional shoot tip culture and shoot tip cryotherapy.
近年来，猕猴桃病毒性疾病在中国猕猴桃产区日益流行，严重影响猕猴桃的产量和品质。这对猕猴桃产业的健康可持续发展构成了重大制约。使用无病毒繁殖材料已被证明是防治植物病毒病最有效的策略。本研究建立了茎尖培养、茎尖低温冷冻和茎尖低温冷冻与热疗相结合的方法，以从中华猕猴桃中根除猕猴桃 A 病毒（AcVA）、猕猴桃 B 病毒（AcVB）和猕猴桃绿斑环斑病毒（AcCRaV）。此外，还评估了茎尖大小对病毒根除的影响。在所确认的三种病毒中，无论采用何种程序，AcVB 最容易根除，其次是 AcVA 和 AcCRaV。与单独的茎尖培养或低温冷冻相比，热疗与茎尖培养或低温冷冻相结合可获得更高的无病毒频率（分别高达 27.3%和 50%）。值得注意的是，热疗与 0.5-1mm 茎尖低温冷冻相结合是从中华猕猴桃中根除病毒最有效的方案，可产生 50%的再生芽，无任何测试病毒。据我们所知，这是首次基于常规茎尖培养和茎尖低温冷冻法从感染多种病毒的猕猴桃中消除病毒的报道。
