Department of Health Informatics, Niigata University of Health of Welfare, Niigata, Japan.
Department of Immunology, Faculty of Medicine, Kindai University, Osaka, Japan.
Eur J Haematol. 2024 Oct;113(4):416-425. doi: 10.1111/ejh.14249. Epub 2024 Jun 10.
Galectin-9 (Gal-9) is an immune checkpoint ligand for T-cell immunoglobulin and mucin domain 3. Although the roles of Gal-9 in regulating immune responses have been well investigated, their biological roles have yet to be fully documented. This study aimed to analyse the expression of Gal-9 bone marrow (BM) cells in C57BL/6J (B6) mice. Furthermore, the co-expression of Gal-9 with the mammalian target of rapamycin (mTOR) and AMP-activated protein kinase (AMPK) was investigated.
The BM cells in adult C57BL/6J (B6) mice were collected and analysed in vitro.
In a flow cytometric analysis of BM cells, Gal-9 was highly expressed in c-KitSca-1CD34CD71 erythroid progenitors (EPs), whereas it was downregulated in more differentiated c-KitCD71TER119 cells. Subsequently, a negative selection of CD3B220Sca-1CD34CD41CD16/32 EPs was performed. This resulted in substantial enrichment of KitCD71Gal-9 cells and erythroid colony-forming units (CFU-Es), suggesting that the colony-forming subset of EPs are included in the KitCD71Gal-9 population. Furthermore, we found that EPs had lower mTOR and AMPK expression levels in Gal-9 knockout B6 mice than in wild-type B6 mice.
These results may stimulate further investigation of the role of Gal-9 in haematopoiesis.
半乳糖凝集素-9（Gal-9）是 T 细胞免疫球蛋白和粘蛋白结构域 3 的免疫检查点配体。虽然 Gal-9 在调节免疫反应中的作用已经得到了很好的研究，但它们的生物学作用尚未得到充分的证明。本研究旨在分析 C57BL/6J（B6）小鼠骨髓（BM）细胞中 Gal-9 的表达。此外，还研究了 Gal-9 与哺乳动物雷帕霉素靶蛋白（mTOR）和 AMP 激活的蛋白激酶（AMPK）的共表达。
从成年 C57BL/6J（B6）小鼠中收集 BM 细胞并进行体外分析。
在 BM 细胞的流式细胞术分析中，Gal-9 在 c-KitSca-1CD34CD71 红细胞系祖细胞（EPs）中高度表达，而在更分化的 c-KitCD71TER119 细胞中表达下调。随后，对 CD3B220Sca-1CD34CD41CD16/32 EP 进行了阴性选择。这导致 KitCD71Gal-9 细胞和红细胞集落形成单位（CFU-E）的大量富集，表明 EP 的集落形成亚群包含在 KitCD71Gal-9 群体中。此外，我们发现 Gal-9 敲除 B6 小鼠的 EPs 中 mTOR 和 AMPK 的表达水平低于野生型 B6 小鼠。
这些结果可能会促使进一步研究 Gal-9 在造血中的作用。
