Jaroenlak Pattana, McCarty Kacie L, Xia Bo, Lam Cherry, Zwack Erin E, Yanai Itai, Bhabha Gira, Ekiert Damian C
Department of Cell Biology, New York University Grossman School of Medicine, New York 10016, USA.
Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
bioRxiv. 2024 May 30:2024.05.30.596468. doi: 10.1101/2024.05.30.596468.
Microsporidia are single-celled intracellular parasites that cause opportunistic diseases in humans. is a prevalent human-infecting species that invades the small intestine. Dissemination to other organ systems is also observed, and is potentially facilitated by macrophages. The macrophage response to infection and the developmental trajectory of the parasite are not well studied. Here we use single cell RNA sequencing to investigate transcriptional changes in both the host and parasite during infection. While a small population of infected macrophages mount a response, most remain transcriptionally unchanged, suggesting that the majority of parasites may avoid host detection. The parasite transcriptome reveals large transcriptional changes throughout the life cycle, providing a blueprint for parasite development. The stealthy microsporidian lifestyle likely allows these parasites to harness macrophages for replication and dissemination. Together, our data provide insights into the host response in primary human macrophages and the developmental program.
微孢子虫是单细胞的细胞内寄生虫，可导致人类发生机会性疾病。 是一种常见的感染人类的物种，可侵入小肠。也观察到其扩散到其他器官系统，巨噬细胞可能促进了这种扩散。巨噬细胞对感染的反应以及寄生虫的发育轨迹尚未得到充分研究。在这里，我们使用单细胞RNA测序来研究感染期间宿主和寄生虫的转录变化。虽然一小部分受感染的巨噬细胞会产生反应，但大多数细胞的转录状态保持不变，这表明大多数寄生虫可能逃避了宿主的检测。寄生虫转录组揭示了其整个生命周期中的大量转录变化，为寄生虫的发育提供了蓝图。微孢子虫这种隐秘的生活方式可能使这些寄生虫利用巨噬细胞进行复制和传播。总之，我们的数据为人类原代巨噬细胞中的宿主反应和 发育程序提供了见解。
