Ombrouck C, Desportes-Livage I, Achbarou A, Gentilini M
INSERM U, Paris, France.
C R Acad Sci III. 1996 Jan;319(1):39-43.
The microsporidia Encephalitozoon intestinalis is an opportunistic pathogen responsible for severe gastrointestinal diseases and disseminated infection in AIDS patients. No light-microscopical method allows the specific detection of this unicellular parasite and up to this date, only electron microscopy could confirm the diagnosis of the species. We propose a method combining the non specific labelling of microsporidian spores by the fluorochrome Uvitex 2B and an indirect immunofluorescent assay with a polyclonal antibody specifically directed against E. intestinalis. Preliminary data demonstrate the specificity of this antibody. This method enables the distinction between E. intestinalis and Enterocytozoon bieneusi an other microsporidian also associated with gastrointestinal infection. Due to the precocious detection of E. intestinalis patients will be treated earlier with albendazole which is potentially active against this species.
微小孢子虫肠道脑炎微孢子虫是一种机会性病原体，可导致艾滋病患者出现严重的胃肠道疾病和播散性感染。目前尚无光学显微镜方法可特异性检测这种单细胞寄生虫，直至今日，只有电子显微镜能够确诊该物种。我们提出一种方法，将荧光染料Uvitex 2B对微孢子虫孢子的非特异性标记与使用特异性针对肠道脑炎微孢子虫的多克隆抗体进行的间接免疫荧光测定相结合。初步数据证明了该抗体的特异性。该方法能够区分肠道脑炎微孢子虫和比氏肠微孢子虫，后者是另一种也与胃肠道感染相关的微孢子虫。由于能够较早检测到肠道脑炎微孢子虫，患者将更早接受对该物种可能有效的阿苯达唑治疗。
