Karlo Jiro, Vijay Arunsree, Phaneeswar Mahamkali Sri, Singh Surya Pratap
Department of Biosciences and Bioengineering, Indian Institute of Technology Dharwad, Dharwad, Karnataka 580011, India.
ACS Omega. 2024 May 22;9(22):23753-23760. doi: 10.1021/acsomega.4c01666. eCollection 2024 Jun 4.
The mode of action of antibiotics can be broadly classified as bacteriostatic and bactericidal. The bacteriostatic mode leads to the arrested growth of the cells, while the bacteriocidal mode causes cell death. In this work, we report the applicability of deuterium stable isotope probing (DSIP) in combination with Raman spectroscopy (Raman DSIP) for discriminating the mode of action of antibiotics at the community level. a well-known model microbe, was used as an organism for the study. We optimized the concentration of deuterium oxide required for metabolic activity monitoring without compromising the microbial growth. Our findings suggest that changes in the intensity of the C-D band in the high-wavenumber region could serve as a quantifiable marker for determining the antibiotic mode of action. This can be used for early identification of the antibiotic's mode of action. Our results explore the new perspective that supports the utility of deuterium-based vibrational tags in the field of clinical spectroscopy. Understanding the antibiotic's mode of action on bacterial cells in a short and objective manner can significantly enhance the clinical management abilities of infectious diseases and may also help in personalized antimicrobial therapy.
抗生素的作用模式大致可分为抑菌和杀菌两种。抑菌模式会导致细胞生长停滞，而杀菌模式则会导致细胞死亡。在这项工作中，我们报告了氘稳定同位素探测（DSIP）与拉曼光谱（拉曼DSIP）相结合在群落水平上区分抗生素作用模式的适用性。一种著名的模式微生物被用作研究对象。我们优化了用于代谢活性监测的氧化氘浓度，同时不影响微生物生长。我们的研究结果表明，高波数区域中C-D带强度的变化可作为确定抗生素作用模式的可量化标记。这可用于早期识别抗生素的作用模式。我们的结果探索了一个新的视角，支持基于氘的振动标签在临床光谱学领域的应用。以简短而客观的方式了解抗生素对细菌细胞的作用模式，可显著提高传染病的临床管理能力，也可能有助于个性化抗菌治疗。
