Schmidt-Melbye A, Kolstad A, Hannestad K
Transfusion. 1985 Mar-Apr;25(2):165-9. doi: 10.1046/j.1537-2995.1985.25285169213.x.
IgG antibodies to neutrophil polymorphonuclear cells (PMNs) were detected by a simple indirect immunofluorescence method that did not require chemical or enzymatic modification of the cells. Based on recent information about the human PMN Fc gamma-receptor, nonspecific binding of IgG to PMNs was virtually completely prevented by incubating cells at 37 degrees C with human serum diluted 1 to 3 in the presence of a high concentration of rabbit IgG. This also facilitated distinction of sera with weak anti-PMN activity from normal sera. On the other hand, nonspecific staining remained on a fraction (10-25%) of mononuclear leukocytes. Following first-time transfusion of 0.5 to 5 units of whole blood or red cell concentrate, 4 of 27 patients (15%) developed anti-leukocyte antibodies. Anti-leukocyte antibodies were detected in 51 percent of 47 patients with reported febrile transfusion reactions; the IgG antibodies bound to PMNs in 38 percent of these 47 patients. Since only about one in three patients with febrile transfusion reactions had detectable IgG anti-PMN antibodies, fever may be caused by lysis of leukocytes other than PMNs.
采用一种简单的间接免疫荧光法检测针对中性粒细胞多形核细胞（PMN）的IgG抗体，该方法无需对细胞进行化学或酶修饰。根据有关人类PMN Fcγ受体的最新信息，通过在37℃下将细胞与人血清以1:3稀释并在高浓度兔IgG存在的情况下孵育，几乎完全防止了IgG与PMN的非特异性结合。这也有助于区分抗PMN活性较弱的血清与正常血清。另一方面，非特异性染色仍存在于一部分（10%-25%）单核白细胞上。在首次输注0.5至5单位全血或红细胞浓缩液后，27例患者中有4例（15%）产生了抗白细胞抗体。在报告有发热性输血反应的47例患者中，51%检测到抗白细胞抗体；在这47例患者中，38%的IgG抗体与PMN结合。由于发热性输血反应患者中只有约三分之一可检测到IgG抗PMN抗体，发热可能是由PMN以外的白细胞裂解引起的。
