Rafiq Muhammad Talha, Sajid Zahoor Ahmad, Khilji Sheza Ayaz
Plant Developmental and Regenerative Biology Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus 54590, Lahore, Pakistan.
Department of Botany, Division of Science and Technology, University of Education Township, Lahore, Pakistan.
Scientifica (Cairo). 2024 May 31;2024:6693085. doi: 10.1155/2024/6693085. eCollection 2024.
Bert. is commonly known as candy leaf, sugar leaf, or sweet leaf. It is a natural sweetener that has low calories and is used as a substitute for sucrose. The objective of this research is to evaluate the effects of graphene oxide (GO) on the growth, biochemical activities, and stevioside and rebaudioside A production of in in raised plantlets. For this, green nanomaterials of GO (0, 2, 4, 6, 8, and 10 mgL) were applied to the plants to enhance its sweetness by triggering the production of stevioside and rebaudioside A and other growth and biochemical parameters. It was observed that all the growth parameters of plants significantly increased with all GO treatments tested. Total chlorophyll and protein contents were increased (1.85- and 2.65-fold increase from the control) by applying 8 mgL of GO to the MS medium. The maximum value (4 mg·g of protein) of peroxidase activity (POD) was observed by applying 4 mgL of GO, 28.92-fold increase from the control. In comparison, superoxide dismutase activity (SOD) (0.4 mg·g protein) was observed with 10 mgL of GO (1.56-fold increase from the control). Stevioside (12.9 and 8.9 mg·g DW) and rebaudioside A (3.2 and 0.81 mg·g DW) were observed only at 6 and 8 mg·L treatment of graphene oxide. According to the findings, using graphene oxide (GO) had a significant impact on the growth, biochemical activities, and steviol glycoside production in . This shows that GO has the potential to be a valuable enhancer of sweetness and overall leaf quality, providing great prospects for the development of low-calorie natural sweeteners.
甜叶菊通常被称为甜叶、糖叶或甜菊叶。它是一种低热量的天然甜味剂，可作为蔗糖的替代品。本研究的目的是评估氧化石墨烯（GO）对甜叶菊组培苗生长、生化活性以及甜菊糖苷和莱鲍迪苷A产量的影响。为此，将不同浓度（0、2、4、6、8和10mg/L）的GO绿色纳米材料施用于甜叶菊植株，以通过触发甜菊糖苷和莱鲍迪苷A的产生以及其他生长和生化参数来提高其甜度。结果发现，所有测试的GO处理均使甜叶菊植株的所有生长参数显著增加。在MS培养基中添加8mg/L的GO可使总叶绿素和蛋白质含量增加（比对照分别增加1.85倍和2.65倍）。添加4mg/L的GO时，过氧化物酶活性（POD）达到最大值（4mg·g蛋白质），比对照增加28.92倍。相比之下，添加10mg/L的GO时超氧化物歧化酶活性（SOD）为（0.4mg·g蛋白质）（比对照增加1.56倍）。仅在6和8mg/L的氧化石墨烯处理下观察到甜菊糖苷（12.9和8.9mg·g干重）和莱鲍迪苷A（3.2和0.81mg·g干重）。根据研究结果，使用氧化石墨烯（GO）对甜叶菊的生长、生化活性和甜菊糖苷产量有显著影响。这表明GO有潜力成为一种有价值的甜度增强剂和整体甜叶菊叶片品质提升剂，为低热量天然甜味剂的开发提供了广阔前景。
