Barkan R S, Nikol'skiĭ N N
Tsitologiia. 1985 Jan;27(1):5-27.
The present review gives a detailed description of mouse cell lines 3T3 (Swiss 3T3, Balb/c 3T3, and NIH 3T3) including their establishment, evolution, growth properties and formation of the specific monolayer with low saturation density. Questionable views of the 3T3 cell origin are discussed. The main features of these continuous cell lines are shown, such as a high requirement for serum growth factors, substrate dependence, inhibition of cell division in dense monolayer and absence of oncogenic potentions, which are similar to those of normal diploid cells, thus making these pseudonormal lines useful in studies of cell proliferation and oncogenesis. Different methods for obtaining quiescent 3T3 cells are observed, and, more in detail, the achievement of the resting state in confluent monolayer and at low serum concentration in sparce culture. The data reporting the efficiency of growth stimulation using serum, purified growth factors and hormones are analysed. Special attention is paid to the susceptibility of 3T3 lines to culture conditions, and to dependence of experimental data obtained upon keeping stability of these conditions.
本综述详细描述了小鼠细胞系3T3（瑞士3T3、Balb/c 3T3和NIH 3T3），包括它们的建立、演变、生长特性以及形成具有低饱和密度的特定单层细胞的过程。文中讨论了关于3T3细胞起源的一些有争议的观点。展示了这些连续细胞系的主要特征，例如对血清生长因子的高需求、对底物的依赖性、在致密单层中细胞分裂的抑制以及无致癌潜能，这些特征与正常二倍体细胞相似，因此使得这些假正常细胞系在细胞增殖和肿瘤发生研究中很有用。观察了获得静止3T3细胞的不同方法，更详细地阐述了在汇合单层以及稀疏培养中低血清浓度下达到静止状态的情况。分析了关于使用血清、纯化生长因子和激素刺激生长效率的数据报告。特别关注3T3细胞系对培养条件的敏感性，以及所获得实验数据对保持这些条件稳定性的依赖性。
