Gilinsky Samuel D, Jung Diane N, Futia Greg L, Zohrabi Mo, Welton Tarah A, Supekar Omkar D, Gibson Emily A, Restrepo Diego, Bright Victor M, Gopinath Juliet T
Department of Electrical, Computer, and Energy Engineering, University of Colorado Boulder, Boulder, Colorado 80309, USA.
Department of Mechanical Engineering, University of Colorado Boulder, Boulder, Colorado 80309, USA.
Biomed Opt Express. 2024 Apr 24;15(5):3285-3300. doi: 10.1364/BOE.516956. eCollection 2024 May 1.
We demonstrate a novel electrowetting liquid combination using a room temperature ionic liquid (RTIL) and a nonpolar liquid, 1-phenyl-1-cyclohexene (PCH) suitable for focus-tunable 3-photon microscopy. We show that both liquids have over 90% transmission at 1300 nm over a 1.1 mm pathlength and an index of refraction contrast of 0.123. A lens using these liquids can be tuned from a contact angle of 133 to 48° with applied voltages of 0 and 60 V, respectively. Finally, a three-photon imaging system including an RTIL electrowetting lens was used to image a mouse brain slice. Axial scans taken with an electrowetting lens show excellent agreement with images acquired using a mechanically scanned objective.
我们展示了一种新型的电润湿液体组合，它使用室温离子液体（RTIL）和非极性液体1-苯基-1-环己烯（PCH），适用于焦点可调谐三光子显微镜。我们表明，两种液体在1300 nm波长下、1.1 mm光程长度上的透过率均超过90%，折射率对比度为0.123。使用这些液体的透镜在分别施加0和60 V电压时，接触角可从133°调至48°。最后，使用包括RTIL电润湿透镜的三光子成像系统对小鼠脑切片进行成像。用电润湿透镜进行的轴向扫描与使用机械扫描物镜获取的图像显示出极佳的一致性。
