用于脑结构三维成像及光学切片的微型结构光照明显微镜。
Miniature structured illumination microscope for 3D imaging of brain structures with optical sectioning.
作者信息
Supekar Omkar D, Sias Andrew, Hansen Sean R, Martinez Gabriel, Peet Graham C, Peng Xiaoyu, Bright Victor M, Hughes Ethan G, Restrepo Diego, Shepherd Douglas P, Welle Cristin G, Gopinath Juliet T, Gibson Emily A
机构信息
Department of Electrical, Energy and Computer Engineering, University of Colorado Boulder, CO 80309, USA.
Department of Mechanical Engineering, University of Colorado Boulder, CO 80309, USA.
出版信息
Biomed Opt Express. 2022 Mar 29;13(4):2530-2541. doi: 10.1364/BOE.449533. eCollection 2022 Apr 1.
We present a high-resolution miniature, light-weight fluorescence microscope with electrowetting lens and onboard CMOS for high resolution volumetric imaging and structured illumination for rejection of out-of-focus and scattered light. The miniature microscope (SIMscope3D) delivers structured light using a coherent fiber bundle to obtain optical sectioning with an axial resolution of 18 µm. Volumetric imaging of eGFP labeled cells in fixed mouse brain tissue at depths up to 260 µm is demonstrated. The functionality of SIMscope3D to provide background free 3D imaging is shown by recording time series of microglia dynamics in awake mice at depths up to 120 µm in the brain.
我们展示了一种高分辨率的微型、轻型荧光显微镜，它配备电润湿透镜和板载互补金属氧化物半导体（CMOS），用于高分辨率体积成像和结构照明显微术，以消除离焦光和散射光。这种微型显微镜（SIMscope3D）使用相干光纤束提供结构光来获得轴向分辨率为18微米的光学切片。展示了对固定小鼠脑组织中深度达260微米的eGFP标记细胞进行体积成像。通过记录清醒小鼠大脑中深度达120微米的小胶质细胞动力学的时间序列，展示了SIMscope3D提供无背景三维成像的功能。
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