Yasaie Shokouh, Mousavi Nasab Seyed Dawood, Shams Saeed, Ferdousi Atousa, Kaghazian Hooman
Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran.
Department of Arboviruses and Viral Hemorrhagic Fevers (National Reference Laboratory), Pasteur Institute of Iran, Tehran, Iran.
Infect Prev Pract. 2024 May 15;6(3):100370. doi: 10.1016/j.infpip.2024.100370. eCollection 2024 Sep.
Gastroenteritis accounts for about 10% of the deaths among children, especially in immunocompromised children. Few studies on the prevalence of gastrointestinal infections caused by RNA viruses have been done in Iran. The aim of the study was to evaluate the detection of RNA viruses causing diarrhoea using a multiplex PCR.
Stool samples were collected from 130 paediatric patients with diarrhoea who had acute lymphocytic leukaemia, non-Hodgkin lymphoma, and retinoblastoma. After RNA extraction and synthesis of cDNA, multiplex PCR was done to evaluate the presence of rotavirus, norovirus, astrovirus, and enterovirus.
There were 9 (6.9%), 7 (5.4%), 3 (2.3%), and 6 (4.6%) cases of rotavirus, norovirus, astrovirus, and enterovirus detected, respectively. One case of co-infection with astrovirus and norovirus was observed.
This is the first report from Iran which identified the presence of common RNA viruses causing diarrhoea in immunocompromised children. Increased awareness of these viruses will enable healthcare professionals to improve strategies and policies to control spread and infection caused by these viruses.
肠胃炎导致儿童死亡的病例约占10%，尤其是免疫功能低下的儿童。伊朗针对RNA病毒引起的胃肠道感染患病率开展的研究较少。本研究旨在评估利用多重聚合酶链反应（multiplex PCR）检测导致腹泻的RNA病毒。
从130名患有急性淋巴细胞白血病、非霍奇金淋巴瘤和视网膜母细胞瘤的腹泻儿科患者中采集粪便样本。提取RNA并合成互补DNA（cDNA）后，进行多重聚合酶链反应，以评估轮状病毒、诺如病毒、星状病毒和肠道病毒的存在情况。
分别检测到9例（6.9%）轮状病毒、7例（5.4%）诺如病毒、3例（2.3%）星状病毒和6例（4.6%）肠道病毒感染病例。观察到1例星状病毒和诺如病毒合并感染病例。
这是伊朗首份关于免疫功能低下儿童中导致腹泻的常见RNA病毒存在情况的报告。提高对这些病毒的认识将使医疗保健专业人员能够改进控制这些病毒传播和感染的策略及政策。
