Key Laboratory of Medical Protection for Electromagnetic Radiation, Ministry of Education, Department of Occupational Health, Army Medical University, Chongqing, China.
Department of Nursing, General Hospital of Northern Theater Command, Shenyang, Liaoning Province, People's Republic of China.
Electromagn Biol Med. 2024 Jul 2;43(3):164-175. doi: 10.1080/15368378.2024.2361873. Epub 2024 Jun 10.
Mild whole-body hyperthermia has been shown to have anti-tumor effects through an immune-modulating mechanism. Before it is widely applied in the clinic, tremendous mechanistic research in animals is necessary to adhere to evidence-based principles. The radio frequency electromagnetic field (RF-EMF) based heating facility could be a good choice for hyperthermia treatment, but the heating characteristics of a facility, including structure design, electromagnetic and thermal dosimetry, and the biologic effects of hyperthermia, need to be well elucidated. Here, we reported the heating characteristic study on a resonant chamber (RC) excited by a 1800 MHz solid source. The EMF in the RC was stirred by 24 static reflectors, which resulted in the standard deviation of electric field intensity being below 3 dB in the EM homogeneity evaluation. For the exposure scenario, six free-moving mice were loaded into separate cases and exposed simultaneously in the RC. The EMF energy absorption and distribution in exposed mice were calculated with the 12-plane-waves method of numerical simulation. Different levels of core body temperature increment in exposed mice were achieved through regulation of the source output power. Overexpression of heat shock proteins (HSPs) was detected in the liver, lung and muscle, but not in the brain of the exposed mice. The levels of representative inflammatory cytokines in the serum, TNF-α and IL-10 increased post RC exposure. Based on the heating characteristic study and validation, the applied RC would be a qualified heating system for mild whole-body hyperthermia effect research in mice.
轻度全身热疗通过免疫调节机制显示出抗肿瘤作用。在将其广泛应用于临床之前，需要在动物中进行大量的机制研究，以遵循循证原则。基于射频电磁场（RF-EMF）的加热设备可能是热疗的一个不错选择，但设施的加热特性，包括结构设计、电磁和热剂量学以及热疗的生物学效应，需要得到充分阐明。在这里，我们报告了用 1800 MHz 固体源激励的谐振腔（RC）的加热特性研究。RC 中的电磁场通过 24 个静态反射器搅拌，导致在电磁场均匀性评估中电场强度的标准偏差低于 3 dB。对于暴露情况，将六只自由移动的老鼠装入单独的容器中并同时在 RC 中暴露。用数值模拟的 12 平面波方法计算了暴露老鼠中的电磁场能量吸收和分布。通过调节源输出功率，实现了暴露老鼠核心体温升高的不同水平。在暴露老鼠的肝脏、肺和肌肉中检测到热休克蛋白（HSPs）的过度表达，但在大脑中没有。暴露后，血清中代表性炎症细胞因子 TNF-α 和 IL-10 的水平增加。基于加热特性研究和验证，所应用的 RC 将成为研究老鼠轻度全身热疗效果的合格加热系统。
