Avdikos Antonios, Karkabounas Spyridon, Metsios Apostolos, Kostoula Olga, Havelas Konstantinos, Binolis Jayne, Verginadis Ioannis, Hatziaivazis George, Simos Ioannis, Evangelou Angelos
Laboratory of Physiology, Unit of Environmental Physiology, Faculty of Medicine, University of Ioannina, Greece.
Hell J Nucl Med. 2007 May-Aug;10(2):95-101.
In the present study, the effects of a resonant low intensity static electromagnetic field (EMF), causing no thermal effects, on Wistar rats have been investigated. Sarcoma cell lines were isolated from leiomyosarcoma tumors induced in Wistar rats by the subcutaneous (s.c) injection of 3,4-benzopyrene. Furthermore, smooth muscle cells (SMC) were isolated from the aorta of Wistar rats and cultivated. Either leiomyosarcoma cells (LSC) or SMC were used to record a number of characteristic resonant radiofrequencies, in order to determine the specific electromagnetic fingerprint spectrum for each cell line. These spectra were used to compose an appropriate algorithm, which transforms the recorded radiofrequencies to emitted ones. The isolated LSC were cultured and then exposed to a resonant low intensity radiofrequency EMF (RF-EMF), at frequencies between 10 kHz to 120 kHz of the radiowave spectrum. The exposure lasted 45 consecutive minutes daily, for two consecutive days. Three months old female Wistar rats were inoculated with exposed and non-exposed to EMF LSC (4 x 10(6) LCS for animal). Inoculated with non-exposed to EMF cells animals were then randomly separated into three Groups. The first Group was sham exposed to the resonant EMF (control Group-CG), the second Group after the inoculation of LSC and appearance of a palpable tumor mass, was exposed to a non-resonant EMF radiation pattern, for 5 h per day till death of all animals (experimental control Group-ECG). The third Group of animals after inoculation of LSC and the appearance of a palpable tumor mass, was exposed to the resonant EMF radiation for 5 h per day, for a maximum of 60 days (experimental Group-I, EG-I). A fourth Group of animals was inoculated with LSC exposed to EMF irradiation and were not further exposed to irradiation (experimental Group-II, EG-II). Tumor induction was 100% in all Groups studied and all tumors were histologically identified as leiomyosarcomas. In the case of the EG-I, a number of tumors were completely regretted (final tumor induction: 66%). Both Groups of animals inoculated with exposed or non-exposed to the EMF LSC, (EG-I and EG-II, respectively) demonstrated a significant prolongation of the survival time and a lower tumor growth rate, in comparison to the control Group (CG) and the experimental control Group (ECG). However, the survival time of EG-I animals was found to be significantly longer and tumor growth rate significantly lower compared to EG-II animals. In conclusion, our results indicate a specific anticancer effect of resonant EMF irradiation. These results may possibly be attributed to (a) the duration of exposure of LSC and (b) the exposure of the entire animal to this irradiation.
在本研究中，对无热效应的共振低强度静态电磁场（EMF）对Wistar大鼠的影响进行了研究。肉瘤细胞系从皮下（s.c）注射3,4-苯并芘诱导的Wistar大鼠平滑肌肉瘤肿瘤中分离得到。此外，从Wistar大鼠的主动脉中分离并培养平滑肌细胞（SMC）。使用平滑肌肉瘤细胞（LSC）或SMC记录一些特征共振射频，以确定每个细胞系的特定电磁指纹谱。这些谱用于构建适当的算法，将记录的射频转换为发射的射频。分离的LSC进行培养，然后暴露于无线电波谱频率在10 kHz至120 kHz之间的共振低强度射频电磁场（RF-EMF）。暴露持续每天连续45分钟，持续两天。将3个月大的雌性Wistar大鼠接种暴露于和未暴露于EMF的LSC（每只动物接种4×10⁶个LCS）。接种未暴露于EMF细胞的动物随后随机分为三组。第一组假暴露于共振EMF（对照组-CG），第二组在接种LSC并出现可触及的肿瘤块后，暴露于非共振EMF辐射模式，每天5小时直至所有动物死亡（实验对照组-ECG）。第三组动物在接种LSC并出现可触及的肿瘤块后，每天暴露于共振EMF辐射5小时，最长60天（实验组-I，EG-I）。第四组动物接种暴露于EMF辐射的LSC且不再进一步暴露于辐射（实验组-II，EG-II）。在所有研究组中肿瘤诱导率均为100%，所有肿瘤经组织学鉴定为平滑肌肉瘤。在EG-I组中，一些肿瘤完全消退（最终肿瘤诱导率：66%）。与对照组（CG）和实验对照组（ECG）相比，接种暴露于或未暴露于EMF的LSC的两组动物（分别为EG-I和EG-II）均表现出存活时间显著延长和肿瘤生长速率降低。然而，发现EG-I组动物的存活时间明显更长，肿瘤生长速率明显低于EG-II组动物。总之，我们的结果表明共振EMF辐射具有特定的抗癌作用。这些结果可能归因于（a）LSC的暴露持续时间和（b）整个动物对这种辐射的暴露。
