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解析细胞质到纤毛运输过程中的囊泡辅助运输机制。

Deciphering vesicle-assisted transport mechanisms in cytoplasm to cilium trafficking.

作者信息

Tingey Mark, Ruba Andrew, Jiang Zechang, Yang Weidong

机构信息

Department of Biology, Temple University, Philadelphia, PA, United States.

出版信息

Front Cell Neurosci. 2024 May 27;18:1379976. doi: 10.3389/fncel.2024.1379976. eCollection 2024.

DOI:10.3389/fncel.2024.1379976
PMID:38860265
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11163138/
Abstract

The cilium, a pivotal organelle crucial for cell signaling and proper cell function, relies on meticulous macromolecular transport from the cytoplasm for its formation and maintenance. While the intraflagellar transport (IFT) pathway has traditionally been the focus of extensive study concerning ciliogenesis and ciliary maintenance, recent research highlights a complementary and alternative mechanism-vesicle-assisted transport (VAT) in cytoplasm to cilium trafficking. Despite its potential significance, the VAT pathway remains largely uncharacterized. This review explores recent studies providing evidence for the dynamics of vesicle-related diffusion and transport within the live primary cilium, employing high-speed super-resolution light microscopy. Additionally, we analyze the spatial distribution of vesicles in the cilium, mainly relying on electron microscopy data. By scrutinizing the VAT pathways that facilitate cargo transport into the cilium, with a specific emphasis on recent advancements and imaging data, our objective is to synthesize a comprehensive model of ciliary transport through the integration of IFT-VAT mechanisms.

摘要

纤毛是细胞信号传导和正常细胞功能的关键细胞器，其形成和维持依赖于从细胞质进行的精确大分子运输。虽然传统上鞭毛内运输（IFT）途径一直是纤毛发生和纤毛维持广泛研究的重点，但最近的研究突出了一种互补且替代的机制——细胞质到纤毛运输中的囊泡辅助运输（VAT）。尽管其具有潜在重要性，但VAT途径在很大程度上仍未得到充分表征。本综述探讨了最近的研究，这些研究利用高速超分辨率光学显微镜为活的初级纤毛内囊泡相关扩散和运输的动态提供了证据。此外，我们主要依靠电子显微镜数据来分析纤毛中囊泡的空间分布。通过仔细研究促进货物运输到纤毛中的VAT途径，特别强调最近的进展和成像数据，我们的目标是通过整合IFT - VAT机制来综合构建纤毛运输的全面模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/852c/11163138/a3d54e52c825/fncel-18-1379976-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/852c/11163138/0273d1890975/fncel-18-1379976-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/852c/11163138/4ebe8be66cad/fncel-18-1379976-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/852c/11163138/7a24190cbc58/fncel-18-1379976-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/852c/11163138/afefa02b746d/fncel-18-1379976-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/852c/11163138/d803fc874a14/fncel-18-1379976-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/852c/11163138/36a0a148db7e/fncel-18-1379976-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/852c/11163138/a3d54e52c825/fncel-18-1379976-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/852c/11163138/0273d1890975/fncel-18-1379976-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/852c/11163138/f2985ef69a53/fncel-18-1379976-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/852c/11163138/4ebe8be66cad/fncel-18-1379976-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/852c/11163138/7a24190cbc58/fncel-18-1379976-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/852c/11163138/afefa02b746d/fncel-18-1379976-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/852c/11163138/d803fc874a14/fncel-18-1379976-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/852c/11163138/36a0a148db7e/fncel-18-1379976-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/852c/11163138/a3d54e52c825/fncel-18-1379976-g008.jpg

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本文引用的文献

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The ciliary lumen accommodates passive diffusion and vesicle-assisted trafficking in cytoplasm-ciliary transport.纤毛腔容纳细胞质纤毛转运中的被动扩散和囊泡辅助运输。
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