Kirshner J, Ozer H, O'Leary M, Higby D, Marinello M, Preisler H
Blut. 1985 Apr;50(4):201-8. doi: 10.1007/BF00320295.
One approach to overcome the problem of histoincompatibility in bone marrow transplantation is to use T cell depleted marrow from a haploidentical donor in an attempt to ameliorate graft-versus-host disease. Since the T cell requirements for normal hematopoiesis are uncertain, experiments were performed to study the effects of E rosette-T cell depletion on in vitro growth of hematopoietic progenitor cells. Marrow mononuclear cells were cultured in a modified CFU-GEMM assay before and after T cell depletion. The number of 7 day granulocytic and erythrocytic colonies, and 14 day granulocytic, erythrocytic and mixed colonies were enumerated and expressed in terms of colonies per 10(5) non T cells plated. T cell depletion did not result in decreased proliferation of any of these progenitors save possibly for 14 day granulocytic colonies in one of four experiments. In two cases, T cell depletion resulted in increased growth of progenitor cells. Three of four patients transplanted with T cell depleted haploidentical marrow cells engrafted. It is concluded that E rosette depletion of T cells from marrow does not decrease the potential of these cells to establish hematopoiesis in vitro or in vivo.
克服骨髓移植中组织不相容问题的一种方法是使用来自单倍体相合供体的T细胞去除的骨髓，以试图改善移植物抗宿主病。由于正常造血所需的T细胞情况尚不确定，因此进行了实验以研究E花环-T细胞去除对造血祖细胞体外生长的影响。在T细胞去除前后，将骨髓单个核细胞在改良的CFU-GEMM测定中培养。对7天的粒细胞和红细胞集落以及14天的粒细胞、红细胞和混合集落的数量进行计数，并以每接种10(5)个非T细胞的集落数表示。除了在四个实验之一中14天的粒细胞集落可能减少外，T细胞去除并未导致这些祖细胞中任何一种的增殖减少。在两个病例中，T细胞去除导致祖细胞生长增加。四名接受T细胞去除的单倍体相合骨髓细胞移植的患者中有三名植入成功。结论是，从骨髓中去除T细胞的E花环法不会降低这些细胞在体外或体内建立造血的潜力。
