Department of Agricultural Entomology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, India.
Department of Plant Molecular Biology and Bioinformatics, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India.
Arch Insect Biochem Physiol. 2024 Jun;116(2):e22123. doi: 10.1002/arch.22123.
Aphids are sap-sucking insects responsible for crop losses and a severe threat to crop production. Proteins in the aphid saliva are integral in establishing an interaction between aphids and plants and are responsible for host plant adaptation. The cotton aphid, Aphis gossypii (Hemiptera: Aphididae) is a major pest of Gossypium hirsutum. Despite extensive studies of the salivary proteins of various aphid species, the components of A. gossypii salivary glands are unknown. In this study, we identified 123,008 transcripts from the salivary gland of A. gossypii. Among those, 2933 proteins have signal peptides with no transmembrane domain known to be secreted from the cell upon feeding. The transcriptome includes proteins with more comprehensive functions such as digestion, detoxification, regulating host defenses, regulation of salivary glands, and a large set of uncharacterized proteins. Comparative analysis of salivary proteins of different aphids and other insects with A. gossypii revealed that 183 and 88 orthologous clusters were common in the Aphididae and non-Aphididae groups, respectively. The structure prediction for highly expressed salivary proteins indicated that most possess an intrinsically disordered region. These results provide valuable reference data for exploring novel functions of salivary proteins in A. gossypii with their host interactions. The identified proteins may help develop a sustainable way to manage aphid pests.
蚜虫是吸食植物汁液的昆虫，会导致作物减产，对作物生产构成严重威胁。蚜虫唾液中的蛋白质在建立蚜虫与植物之间的相互作用中起着至关重要的作用，并且负责宿主植物的适应。棉蚜，Aphis gossypii（半翅目：蚜科）是棉花的主要害虫。尽管对各种蚜虫唾液蛋白进行了广泛的研究，但棉蚜唾液腺的成分仍不清楚。在这项研究中，我们从棉蚜的唾液腺中鉴定出了 123,008 个转录本。在这些转录本中，有 2933 种蛋白质具有信号肽，没有跨膜结构域，已知在进食时从细胞中分泌出来。转录组包括具有更全面功能的蛋白质，如消化、解毒、调节宿主防御、调节唾液腺以及大量未被表征的蛋白质。不同蚜虫和其他昆虫与棉蚜唾液蛋白的比较分析表明，183 个和 88 个直系同源簇分别在蚜科和非蚜科组中常见。高度表达的唾液蛋白的结构预测表明，大多数都具有无规则区域。这些结果为探索唾液蛋白在与宿主相互作用中的新功能提供了有价值的参考数据。鉴定出的蛋白质可能有助于开发可持续的方法来管理蚜虫害虫。
