分光光度法测定羟丙酮酸还原酶活性的研究。
Spectrophotometric Assays for Measuring Hydroxypyruvate Reductase Activity.
机构信息
Biology Department, Eastern Michigan University, Ypsilanti, MI, USA.
出版信息
Methods Mol Biol. 2024;2792:77-81. doi: 10.1007/978-1-0716-3802-6_6.
PMID:38861079
Abstract
Hydroxypyruvate reductase (HPR; EC 1.1.1.81) activity is integral to the photorespiratory pathway. Within photorespiration, HPR catalyzes the reduction of hydroxypyruvate, a product of the serine:glyoxylate aminotransferase reaction to glycerate, a substrate for glycerate kinase, using NADH as cofactor. Here we detail a spectrophotometric assay for measuring HPR activity in vitro by following the consumption of NADH at 340 nm.
摘要
羟丙酮还原酶（HPR；EC 1.1.1.81）的活性是光呼吸途径所必需的。在光呼吸中，HPR 催化羟丙酮的还原，羟丙酮是丝氨酸：乙醛酸转氨酶反应的产物，生成甘油酸，甘油酸是甘油酸激酶的底物，使用 NADH 作为辅助因子。在这里，我们详细介绍了一种分光光度法测定 HPR 体外活性的方法，通过在 340nm 处监测 NADH 的消耗来进行。
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