头颈部癌症患者中细胞外体 miRNAs 及其相关基因的失调。
Dysregulation of exosomal miRNAs and their related genes in head and neck cancer patients.
机构信息
Cancer Genetics & Epigenetics research group, Department of Biosciences, COMSATS University, Park Road Islamabad, Pakistan.
出版信息
Future Oncol. 2024;20(21):1479-1493. doi: 10.1080/14796694.2024.2351355. Epub 2024 May 30.
The present study aimed to figure out the potential role of exosomal microRNAs, and their targeted genes in HNC detection/diagnosis. In the present study, exosomes were extracted from the serum samples of 400 HNC patients and 400 healthy controls. Exosomes were characterized using TEM, NTA, TEM-immunogold labeling and ELISA. Quantitative PCR was used to measure the expression level of exosomal miRNA-19a, miRNA-19b and targeted genes and in HNC patients and controls. The deregulation of miR-19a (p < 0.01), miR-19b (p < 0.03), (p < 0.04) and (p < 0.04) was observed in HNC patients vs controls. ROC curve and Kaplan-Meier analysis showed the good diagnostic/prognostic value of selected exosomal microRNAs and related genes in HNC patients.
本研究旨在探讨外泌体 microRNAs 及其靶基因在头颈部癌症（HNC）检测/诊断中的潜在作用。本研究从 400 例 HNC 患者和 400 例健康对照者的血清样本中提取外泌体。使用 TEM、NTA、TEM-免疫金标记和 ELISA 对其进行鉴定。采用 qPCR 检测 HNC 患者和对照组中外泌体 miRNA-19a、miRNA-19b 及靶基因 和 的表达水平。结果显示，HNC 患者中 miR-19a（p<0.01）、miR-19b（p<0.03）、 （p<0.04）和 （p<0.04）表达下调。ROC 曲线和 Kaplan-Meier 分析表明，所选外泌体 microRNAs 和相关基因对头颈部癌症患者具有良好的诊断/预后价值。
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