Department of Biosciences, Cancer Genetics & Epigenetics Research Group, COMSATS University Islamabad, Pakistan.
Department of Rheumatology, National University of Medical Sciences, Rawalpindi, Pakistan.
Future Oncol. 2023 Jul;19(22):1563-1576. doi: 10.2217/fon-2023-0234. Epub 2023 Aug 14.
The current study was designed to evaluate the diagnostic significance of the exosomal miRNAs and and the gene in brain tumor patients versus controls. Exosomes were extracted from the serum samples of 400 brain tumor patients and 400 healthy controls. The exosomes were characterized by scanning electron microscopy, dynamic light scattering and ELISA. Quantitative PCR was used to analyze selected exosome miRNAs and gene expression levels. Analysis showed significant deregulated expression of (p < 0.0001), (p < 0.0001) and (p < 0.001) in patients versus controls. Spearman correlation showed a significant correlation among the selected exosomal miRNAs and the gene. Receiver operating characteristic curve analysis showed the good diagnostic value of exosomal miRNAs and the gene in brain tumor patients.
本研究旨在评估外泌体 miRNA 和 基因在脑肿瘤患者与对照组中的诊断意义。从 400 例脑肿瘤患者和 400 例健康对照者的血清样本中提取外泌体。通过扫描电子显微镜、动态光散射和 ELISA 对其进行表征。采用定量 PCR 分析选定的外泌体 miRNA 和基因表达水平。分析显示，患者与对照组相比， （p<0.0001）、 （p<0.0001）和 （p<0.001）表达显著下调。Spearman 相关性分析显示，所选外泌体 miRNA 和 基因之间存在显著相关性。受试者工作特征曲线分析显示，外泌体 miRNA 和 基因对脑肿瘤患者具有良好的诊断价值。
