Protocol for measuring cell cycle Zn dynamics using a FRET-based biosensor.

The exchangeable Zn pool in cells is not static but responds to perturbations as well as fluctuates naturally through the cell cycle. Here, we present a protocol to carry out long-term live-cell imaging of cells expressing a cytosolic Zn sensor. We then describe how to track cells using the published pipeline EllipTrack and how to analyze the single-cell traces to determine changes in labile Zn in response to perturbation. For complete details on the use and execution of this protocol, please refer to Rakshit and Holtzen et al..