Grimm Jonathan B, Muthusamy Anand K, Liang Yajie, Brown Timothy A, Lemon William C, Patel Ronak, Lu Rongwen, Macklin John J, Keller Philipp J, Ji Na, Lavis Luke D
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
Nat Methods. 2017 Oct;14(10):987-994. doi: 10.1038/nmeth.4403. Epub 2017 Sep 4.
Pushing the frontier of fluorescence microscopy requires the design of enhanced fluorophores with finely tuned properties. We recently discovered that incorporation of four-membered azetidine rings into classic fluorophore structures elicits substantial increases in brightness and photostability, resulting in the Janelia Fluor (JF) series of dyes. We refined and extended this strategy, finding that incorporation of 3-substituted azetidine groups allows rational tuning of the spectral and chemical properties of rhodamine dyes with unprecedented precision. This strategy allowed us to establish principles for fine-tuning the properties of fluorophores and to develop a palette of new fluorescent and fluorogenic labels with excitation ranging from blue to the far-red. Our results demonstrate the versatility of these new dyes in cells, tissues and animals.
推动荧光显微镜技术的前沿发展需要设计具有精细调控特性的增强型荧光团。我们最近发现，将四元氮杂环丁烷环引入经典荧光团结构会显著提高亮度和光稳定性，从而产生了Janelia Fluor（JF）系列染料。我们改进并扩展了这一策略，发现引入3-取代氮杂环丁烷基团能够以前所未有的精度合理调控罗丹明染料的光谱和化学性质。这一策略使我们能够确立微调荧光团性质的原则，并开发出一系列新的荧光和荧光生成标签，激发范围从蓝光到远红光。我们的结果证明了这些新染料在细胞、组织和动物中的多功能性。
